氟化钠对成骨细胞内Gs/Gi-AC-PKA信号途径的影响  

Expression of Gs/Gi-AC-PKA signal transduction pathway in osteoblast treated with sodium fluoride

作　　者：王楠兰 吴彦秋[1] 杨瑶[1] 曾贝贝[1] 张华[1] 韦艳[1] 

机构地区：[1]贵州医科大学公共卫生学院环境卫生学教研室,环境污染与疾病监控省部共建教育部重点实验室,贵州贵阳550004

出　　处：《现代预防医学》2017年第9期1676-1680,共5页Modern Preventive Medicine

基　　金：国家自然科学基金(81560515);贵州省科技厅社会发展攻关计划项目(黔科合SY字[2013]3062号);科技部国家科技支撑计划课题(2013BAI05B03)

摘　　要：目的探讨氟化钠(NaF)对大鼠成骨骨肉瘤UMR-106细胞及小鼠MC3T3-E1成骨细胞内Gs/Gi-AC-PKA信号途径的影响。方法以不同剂量NaF染毒UMR-106和MC3T3-E1细胞,培养24 h,用CCK-8法测定细胞增殖活性;用qPCR和Western Blot分别测定刺激性G蛋白(Stimulating adenylate cyclase g protein,Gs)、抑制性G蛋白(inhibitory adenylate cyclase g protein,Gi)、腺苷酸环化酶(adenylate cyclase,AC)和蛋白激酶A(protein kinase A,PKA)的mRNA和蛋白的表达水平。结果 UMR-106细胞和MC3T3-E1细胞NaF染毒后的存活率随NaF浓度增加而降低(P<0.05);不同剂量组之间,UMR-106细胞和MC3T3-E1细胞Gs、Gi mRNA的表达差异有统计学意义(P<0.05),且随着染毒剂量的升高呈先升高后下降趋势;AC mRNA表达及蛋白表达差异均有统计学意义(P<0.05),且呈明显下调趋势;UMR-106细胞和MC3T3-E1细胞的PKA mRNA和蛋白的表达差异有统计学意义(P<0.05),且随着染毒剂量的增加而呈明显上升趋势。结论 Gs/Gi-AC-PKA信号途径在氟骨症的形成过程中可能发挥一定作用。Objective To explore the effect of excessive Sodium fluoride （NaF） on Signal Transduetion Pathway Gs/Gi - AC - PKA mRNA expression in Rat osteosarcoma UMR - 106 cells and Mouse osteosarcoma MC3TC - E1 cells. Methods UMR - 106 cells and MC3T3 - E1 cells were exposed to different NaF solution for 24h. CCK - 8 assay was used to measure cell viability. Gs, Gi,AC,PKA mRNA were examined by the methods of real - time PCR. Results The expression of Gs, Gi, AC and PKA UMR - 106 cells and MC3T3 - E1 cells increased at first and then decreased. There were significant differences in AC mRNA expression and protein expression between different dose groups （ P 〈 0.05 ）, and there was a trend of down - regulation. The expression of PKA mRNA and protein in UMR- 106 cells and MC3T3 -E1 cells were significantly different（ P 〈 0.05 ）, and increased with the dose. Conclusion The Gs/Gi - AC - PKA signalling pathway may play a catalytic role in the mechanism of skeletal fluorosis.

关 键 词：氟化钠 UMR-106细胞 ME3T3-E1细胞 Gs/Gi-AC-PKA信号通路 

分 类 号：R113[医药卫生—公共卫生与预防医学]