离体叶片接种法鉴定大豆疫霉根腐病抗病性  

Resistance Identification of Soybean Sources to Phytophthora Root Rot by Leaf Inoculation Method

作　　者：刘淼[1,2] 来永才[1] 李炜[1] 毕影东[1,3] 刘明[1,2] 徐鹏飞[4] 李琬[1,3] 王玲[1] 邸树峰[1] 丁俊男[2] 

机构地区：[1]黑龙江省农业科学院耕作栽培研究所,黑龙江哈尔滨150086 [2]黑龙江省农业科学院博士后工作站,黑龙江哈尔滨150086 [3]中国科学院北方粳稻分子育种联合研究中心,黑龙江哈尔滨150086 [4]东北农业大学大豆生物学教育部重点实验室,农业部东北大豆生物学与遗传育种重点实验室,黑龙江哈尔滨150030

出　　处：《安徽农业科学》2017年第11期43-46,共4页Journal of Anhui Agricultural Sciences

基　　金：黑龙江省青年科学基金项目(QC2014C030);哈尔滨市应用技术研究与开发项目(2014RFQYJ020)

摘　　要：[目的]探讨离体叶片接种法鉴定野生大豆抗病性的准确性和可靠性。[方法]利用24份栽培大豆对离体叶片接种法和下胚轴伤口接种法的可行性进行考察,并利用离体叶片接种法对177份黑龙江省采集的野生大豆资源进行了抗病性鉴定。[结果]离体叶片接种法与下胚轴接种法之间的相关呈极显著,且2种方法差异不显著,证明了离体叶片接种法进行大豆疫霉根腐病抗性鉴定同样准确可行。通过离体叶片接种法对177份黑龙江省采集的野生大豆资源的抗病性鉴定,获得27份抗病材料。[结论]离体叶片接种法操作简单,适合野生大豆抗病性鉴定。[ Objective] To explore accuracy and reliability of resistance identification of cultivated soybean sources to Phytophthora root rot by leaf inoculation method. [ Method] We studied the accuracy of resistance identification of 24 soybean sources to Phytophthora root rot by leaf inoculation method and hypocotyl wound inoculation method, and identified 177 G. soja varieties from Heilongjiang by leaf inoculation method. [ Result] There was significan~ correlation between leaf inoculation and hypocotyl inoculation method, and there were no significant differences between the two methods. At same time, 177 G. soja varieties from Heilongjiang were valuated the reactions tO P. sojae race 1 by way of leaf inoculation. Of all the varieties tested, 27 were resistant. [ Conclusion] The leaf inoculation is easy to operate and suitable for the resistant identification of wild soybean.

关 键 词：野生大豆 大豆疫霉根腐病 抗病鉴定 叶片 

分 类 号：S565.1[农业科学—作物学]