河南省猪等孢球虫的分子变异规律研究  

Study on the Molecular Variation Laws of Isospora suis in Henan Province

作　　者：刘光辉[1] 程俊贞[2] 宁长申[3] 

机构地区：[1]河南省动物疫病预防控制中心,河南郑州450008 [2]许昌市动物疫病预防控制中心,河南许昌461000 [3]河南农业大学牧医工程学院,河南郑州450002

出　　处：《安徽农业科学》2017年第11期93-95,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]掌握河南省猪等孢球虫的分子变异规律。[方法]从河南省豫东、豫南、豫西、豫北及豫中地区的部分规模化猪场采集粪便样品,进行猪球虫卵囊的分离与纯化;从分离到的8株猪球虫分离株中提取核酸并进行测序,并与猪等孢球虫扩增株基因序列及其他相关原虫基因序列进行比对。[结果]河南省不同地区的8个猪球虫株均为猪等孢球虫,并且没有明显遗传差异。I.suis与其他7种原虫差异较大,不属于同一个生物型,同一种动物所感染的不同种类原虫之间的同源性较低。[结论]该研究结果可为有效预防和控制仔猪球虫病提供科学依据。[ Objective ] To master the molecular variation laws of Isospora suis in Henan Province. [ Method ] The pig manure samples were collected from some large-scale pig faims in eastern, western, southern, northern and central regions of Henan Province to isolate and purify porcine coccidian oocysts. 8 isolated strains of swine coccidiosis were used to extract nucleic acid and sequence. The gene sequence of L suis were compared with that of other related protozoon. [ Result] 8 swine coccidian strains from different regions of Henan Province were identified as L su/s and there was no obvious genetic differences. I. suis had greater differences with other 7 kinds of protozoon. L suis and other 7 kinds of protozoon belonged to different biotypes. -The homology of different kinds of protozoon infected in the same kinds of animals was lower. [ Conclusion] The research results can provide scientific basis for effective prevention and control of piglet coceidiosis.

关 键 词：猪等孢球虫 纯化 扩增 基因序列 

分 类 号：S858.28[农业科学—临床兽医学]