单唾液酸四己糖神经节苷脂钠大鼠在体循环肠灌流吸收探究  

Study on the absorption of monosialotetrahexosylganglioside sodium in rats with in situ cycle intesti-nal perfusion methods

作　　者：邓龙 王瑶 陈美玲 熊英 罗嘉琪 谭淑凤 吴伟 周文 姜德建 王小青 

机构地区：[1]湖南省药物安全评价研究中心,新药药效与安全性评价湖南省重点实验室,长沙410331 [2]珠海赛隆药业股份有限公司长沙医药研发中心,长沙410000

出　　处：《中国医师杂志》2017年第4期510-513,共4页Journal of Chinese Physician

摘　　要：目的研究单唾液酸四己糖神经节苷脂钠（GMl）在大鼠十二指肠的吸收动力学特征。方法采用大鼠在体肠循环灌流装置，以GMl为考察指标，酚红为标示物，分别采用紫外分光光度法（uV法）和高效液相色谱法（HPLC法）检测酚红、GMl在大鼠在体肠循环灌流液中的含量。结果GMl在十二指肠循环灌流吸收率：循环2h后吸收率约为10％，循环6h后吸收率约为22％；循环8h的吸收速率常数为（0．030±0．012）h，吸收半衰期t。为（25．50±8．56）h。结论GMl在大鼠十二指肠可以吸收；在十二指肠药物累积吸收量与循环时间基本呈线性关系，可初步判断其吸收动力学为一级动力学过程。Objective To investigate the duodenum absorptive character of monosialotetrahexosyl- ganglioside sodium （GMI） in rats. Methods The contents of phenolsulfonphthalein （as indicators） and GM1 were determined with ultraviolet-visible （UV） method and high performance liquid chromatography （HPLC） method in rats with in situ cycle intestinal perfusion model. Results The ratio of duodenum ab- sorption of GM1 was 10% in 2 h after cycle and 22% in 6 h after cycle, respectively. The Ka was （0. 030 ±0. 012）h, and absorption tl/2 was （25.50± 8.56 ）h in 8 h after cycle. Conclusions GM1 is absorption in rat duodenum, and the accumulate absorption of GM1 is almost linearly related to the cycle time. The ab- sorption dynamics of GM1 may be first-order kinetic process.

关 键 词：G(M1)神经节苷脂/药代动力学 肠吸收 灌流 

分 类 号：R965[医药卫生—药理学]