依达拉奉对兔持续受压节段脊髓神经细胞凋亡的影响  

An experimental study about how edaravone affect the nerve cells apoptosis in the compressed spinalcord

作　　者：高博[1] 董明岩 丁绪元[3] 刘玉林[1] 王国喜[1] 付文举[1] 郭阳[1] 孙产海 

机构地区：[1]泰州市第二人民医院骨科,江苏省泰州225500 [2]锦州医科大学附属第一医院骨科,辽宁省锦州121000 [3]泰州市第二人民医院神经外科,江苏省泰州225500

出　　处：《中国医师杂志》2017年第4期514-517,共4页Journal of Chinese Physician

基　　金：辽宁省科技厅项目（2013225305）

摘　　要：目的浅析依达拉奉对颈脊髓受压节段神经元凋亡的影响。方法组I（空白对照）、组Ⅱ（依达拉奉治疗）两组各150只兔子。颈后正中入路金属螺钉持续压迫颈6和颈7间隙脊髓，模拟脊髓持续轻度受压。实验动物颈段脊髓受压6h后，组Ⅱ经实验动物耳缘血管注射依达拉奉30nag，组I仅注射同等量的等渗NaCI溶液。组I、组Ⅱ再分为3个亚组，每组50只，分别受压1、3、7d，每亚组达到受压时长后即行受压段减压，分别于减压前、减压后1、3、7、14d五个时间点取10只实验动物进行实验，进行动物脊髓损伤评分（BBB）、评估受压节段颈髓神经细胞凋亡情况。结果（1）脊髓受压阶段：两组受压1、3d的兔子，减压前BBB评分、细胞凋亡率、Bax蛋白表达比较差异有统计学意义（P〈0．05），受压7d的两组兔子各项指标差异无统计学意义（P〉0．05）。（2）脊髓减压阶段：两组受压1、3d的兔子，减压1、3、7d时间点BBB评分、细胞凋亡率、Bax蛋白表达比较差异均有统计学意义（P〈0．05），减压14d时各指标差异无统计学意义（P〉0．05）；两组受压7d的兔子，在减压各时间点各指标组问比较差异均无统计学意义（P〉0．05）。结论依达拉奉对颈髓短时间受压（本实验中〈3d）有保护性作用，并可以改善颈髓减压后神经功能恢复。Objective To investigate the protective function of edaravone in the compressed spinal cord. Methods There were 150 rabbits enrolled in each group in the experiment. Rabbits in both opera- tion group and edaravone （EDA） treating group received mild spinal cord compressionby setting a flap head screw between C6 C7 after the neck. The spinal cord decompression was conducted seven days later. After 6 hours, rabbits in the EDA treating group were injected with a large amount of EDA through ear border veins, while the rabbits in the operation group only received 0. 9% sodium chloride injection. The transmis- sion electron microscope was used to observe the apoptotic bodies at 1 day, 3 days and 7 days after compres- sion, and 1 day, 3 days, 7 days, and 14 days after decompression. Flow cytometry was used to test the rate of apoptosis of spinal cord ceils. Immunohistochemistry was used to test the expression of Bax protein that is related to apoptosis. Results The neuronal apoptosis appeared after compression in both operation group and EDA-treating group. The Basso Beattie Bresnahan （BBB） score, neuronal apoptosis rates, and Bax protein expressions in both groups were statistically different （ P 〈 0. 05 ） when the spinal cord was com- pressed in the first day and the third day, while there was no statistically different when spinal cord com- pressed at the seventh day （P 〉 0. 05）. After decompression of the spinal cord, the BBB score, neuronalapoptosis rates, and Bax protein expressions in both groups were becoming lower at the seventh day （ P 〈 0. 05）. Conclusions EDA has protective function for compressed spinal cord. However, only the com- pression of spinal cord compression period of sufficient decompression can fundamentally protect the spinal cord.

关 键 词：安替比林/类似物和衍生物/药理学 脊髓压迫症 脊髓/细胞学/病理学/药物作用 神经元/病理学/药物作用 细胞凋亡 

分 类 号：R651.2[医药卫生—外科学]