黄芪降低糖尿病大鼠出现胰岛素抵抗风险的研究  

Radix Astragali decreases the risk of insulin resistance in insulin-treated diabetic rats through lower-ing oxidative stress

作　　者：熊书晗 李云[2] 孟繁竹[1] 任淑萍[1] 

机构地区：[1]吉林大学公共卫生学院劳动卫生与环境卫生教研室,吉林省长春130021 [2]吉林大学中日联谊医院眼科,吉林省长春130033

出　　处：《中国医师杂志》2017年第4期552-555,共4页Journal of Chinese Physician

摘　　要：目的探讨黄芪对糖尿病动物应用胰岛素治疗出现胰岛素抵抗的作用。方法应用STZ建立糖尿病大鼠模型。将糖尿病模型大鼠分为糖尿病组（DM），黄芪组（RA），胰岛素组（Ins），黄芪＋胰岛素组（RA＋Ins），每组10只大鼠。同时设立正常大鼠为对照组。DM组大鼠给予PBS灌胃；Ins组大鼠给予肌肉注射胰岛素，注射剂量根据血糖浓度进行调整，保持血糖浓度维持在8mmol／L；RA组大鼠给予RA灌胃，剂量为10∥kg；Ins＋RA组大鼠给予胰岛素和RA灌胃剂量同上。连续给药60d，60d后分别采用ELISA法测定肾脏的糖基化终末产物（AGE）水平、血清中TNF—d和IL-6水平，Westernblot检测骨骼肌中IRS-1、p-JNK、p-IRS—Ser307、p-IRS-1Tyr612的表达。结果各组大鼠的肾脏中AGE水平显著高于正常组（P〈0．05），RA＋Ins组肾脏AGE水平显著低于RA组和Ins组（P〈0．05）；RA＋Ins组大鼠血清中的TNF—d和IL-6水平显著低于RA组和Ins组（P〈0．05），而骨骼肌中IRS-1的表达显著高于RA组和Ins组（P〈0．05），P—JNKThrl83和Tyrl85及p-p38MARK的表达均显著低于RA组和Ins组（P〈0．05）。结论糖尿病大鼠在应用胰岛素治疗的同时进行抗氧化治疗能显著降低发生胰岛素抵抗的风险。Objective Fluctuation of glucose levels is more likely to cause oxidative stress which contributes to the development of insulin resistance through activating c-Jun N-terminal kinase （JNK）. Such antio xidants as vitamin C or vitamin E do not appear very helpful. Radix Astragali （RA） is an herbal medi- cine with antioxidative ability. The study was to explore whether RA would lower the risk of insulin resist- ance in diabetic rats. Methods Diabetic rats received RA, insulin or both RA and insulin after diabetes were induced in male Wistar rats. Serum levels of interleukin 6 （IL-6） and tumor necrosis factora （TNF- a）, advanced glycation endproducts（AGEs） levels in kidney, the expression of insulin receptor （IR）, in- sulin receptor substrate l （ IRS-1 ）, p38 mitogen-activated protein kinase （MAPK） , p-p38 MAPK, p-JNK, p-IRS-1 Ser307 , and p-IRS-1 Tyr612 in skeletal muscles were determined. Results Compared to diabetic rats treated with insulin, the diabetic rats treated with both insulin and RA demonstrated significantly lower levels of IL-6, TNF-a and AGEs （ P 〈 0. 05 ）, significantly lower activation of p-p38 MAPK and JNK （ P 〈 0.05）, significantly higher expressions of IRS-I （ P 〈 0.05）, p-IRS-1 Tyr612, and significantly lower ex- pression of p-IRS-1 Ser307 （P 〈 0. 05）. Conclusions RA can lower the risk of insulin resistance through fighting oxidative stress in diabetic rats.

关 键 词：黄芪△/药理学 糖尿病 胰岛素抗药性 

分 类 号：R285.5[医药卫生—中药学]