用于SRAP分析的大豆DNA快速提取方法  

Rapid Isolation of Soybean DNA for SRAP Analysis

作　　者：王芳 李领川 周延清[2] 

机构地区：[1]郑州工程技术学院化工食品学院,郑州450044 [2]河南师范大学生命科学学院,河南新乡453007

出　　处：《中州大学学报》2017年第2期113-116,共4页Journal of Zhongzhou University

基　　金：河南省教育厅自然科学研究计划项目(2009B180016)

摘　　要：以大豆干种子为实验材料,采用改良的CTAB法对大豆DNA进行提取,用琼脂糖电泳进行检测,并用聚丙烯凝胶电泳进行SRAP分子标记的PCR扩增。实验结果表明,该方法提取的NDA条带清晰,质量好,适用于SRAP分子标记的PCR扩增,为进行大豆SRAP遗传多样性的研究奠定了基础。A method of rapid DNA extraction from dry soybean seeds was established based on improved C T A B procedure. Agarose electrophoresis method and SRAP - PCR technology were used to test the DNA . The results showed that the DNA extracted with this method with clear and good quality,applied to SRAP - PCR , and also laid a solid foundation for the research on genetic diversity of soybean.

关 键 词：大豆 SRAP DNA提取 

分 类 号：S565.1[农业科学—作物学]