两种麻醉方法对大鼠蛛网膜下腔阻滞模型建立的影响  

Effects of isoflurane and pentobarbital on the establishment of subarachnoid block model in rats

作　　者：汪芳俊[1] 胡建华[1] 杨宇焦 万勇[1] 

机构地区：[1]川北医学院附属医院麻醉科,四川南充637000

出　　处：《西安交通大学学报（医学版）》2017年第3期462-465,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：四川省科技厅课题(No.2011JYZ024)~~

摘　　要：目的比较大鼠异氟烷吸入麻醉与戊巴比妥钠腹腔注射麻醉对大鼠蛛网膜下腔阻滞模型建立的影响。方法将4月龄SD大鼠60只随机分成A、B两组,每组30只。A组大鼠腹腔注射10g/L戊巴比妥钠溶液30mg/kg,术中根据需要追加首次剂量的1/4;B组采用吸入麻醉箱对大鼠进行麻醉诱导,并采用大鼠面罩吸入异氟烷维持麻醉,术中根据麻醉深度调节吸入麻醉药的浓度。记录两组大鼠麻醉诱导时间、手术时间、麻醉时间、苏醒时间和术中麻醉效果,观察两组大鼠麻醉前后血氧饱和度、呼吸频率、心率,体温变化情况,以及两组大鼠麻醉死亡和建模成功情况。结果两组大鼠月龄、体质量、术中血氧饱和度和体温比较差异无统计学意义(P>0.05)。麻醉后1~60min,A组大鼠心率较B组显著降低(P<0.05);麻醉后5min,A组大鼠呼吸频率较B组显著降低(P<0.05);与A组比较,B组麻醉诱导时间、麻醉维持时间、手术时间和麻醉苏醒时间均显著缩短(P<0.05);术中B组麻醉效果明显较A组好(P<0.05);B组大鼠建模成功率明显较A组高,而麻醉死亡率较A组低(P<0.05)。结论大鼠异氟烷吸入麻醉具有操作简单、麻醉起效快、术中麻醉维持平稳、术后麻醉苏醒迅速等特点,能为大鼠蛛网膜下腔阻滞模型建立提供良好的麻醉效果。Objective To compare the effects of isoflurane and pentobarbital on the establishment of subarachnoid block model in rats.Methods 60 SD rats aged 4 months were randomly divided into Group A(n=30)and Group B(n=30).Rats in Group A received intraperitoneal injection of 10 g/L pentobarbital sodium solution 30 mg/kg and 1/4 of the initial dosage was added according to the operation effect.The induction and maintenance of anesthesia were achieved by isoflurane inhalation in Group B during operation.We recorded the time of anesthesia induction,quality of anesthesia,time of anesthesia,time of operation,and recovery time.The heart rate,respiration frequency,temperature,and saturation of blood oxygen were recorded during operation.We compared death from anesthesia and success of modeling in the two groups.Results There was no significant difference between the groups with regard to age,weight,body temperature or saturation of blood oxygen(P>0.05).Compared to Group B,heart rate decreased 1-60 minutes after anesthesia and respiration frequency decreased 5 minutes after anesthesia in Group A(P<0.05).The time of anesthesia induction,time of anesthesia,time of operation,and recovery time were shorter in Group B(P<0.05).The quality of anesthesia was better in Group B(P<0.05).The success rate of modeling was higher but mortality rate of anesthesia was lower in Group B than in Group A(P <0.05).Conclusion Compared with intraperitoneal injection of pentobarbital sodium,isoflurane inhalation can provide a better anesthetic effect during the operation to establish a rat model of subarachnoid block.

关 键 词：大鼠 异氟烷 吸入麻醉 戊巴比妥钠 腹腔注射 蛛网膜下腔阻滞模型 

分 类 号：Q95-33[生物学—动物学]