BIIB021对MDS细胞株Skm-1增殖的多靶点抑制作用研究  

BIIB021 inhibits proliferation of myelodysplastic syndrome Skm-1 cells and its mechanism

作　　者：李静[1] 沈益民[1] 林圣云[1] 陈珍珍[1] 

机构地区：[1]浙江医院急诊科,杭州310013

出　　处：《浙江医学》2017年第8期599-600,605,共3页Zhejiang Medical Journal

摘　　要：目的探讨热休克蛋白90(HSP-90)抑制剂BIIB021对骨髓增生异常综合征(MDS)细胞株Skm-1增殖的多靶点抑制作用。方法分别将0、100、200、400nmol/L BIIB021作用于4组Skm-1细胞(对照组、100 nmol/L组、200 nmol/L组、400 nmol/L组),培养24h后采用Western blot法检测各组细胞雷帕霉素靶蛋白(mTOR)、mTOR复合物1(mTORC1)、低氧诱导因子-1α(HIF-1α)蛋白表达水平。结果各组Skm-1细胞mTOR蛋白表达水平比较差异无统计学意义(P>0.05)。各组Skm-1细胞mTORC1、HIF-1α蛋白表达水平比较差异均有统计学意义(均P<0.05);组间两两比较差异亦均有统计学意义(均P<0.05),即100nmol/L组、200 nmol/L组、400 nmol/L组Skm-1细胞mTORC1、HIF-1α蛋白表达水平均低于对照组,且400 nmol/L组表达水平最低。结论 BIIB021通过抑制mTORC1和HIF-1α蛋白的表达抑制Skm-1细胞增殖。Objective To investigate the effect of HSP-90 inhibitor BIIB021 on the proliferation of myelodysplasticsyndrome（MDS） Skm-1 cells and related mechanisms. Methods Skm-1 cells were treated with 100, 200 and 400nmol/l BIIB021for 24h. The expression of proteins mTOR, mTORC1, HIF-1α in Skm-1 cells was examined by Western blot. Results Theexpression of protein mTOR in Skm-1 cells was not significantly changed after treatment with different concentrations of BIIB021（P 〉0.05）. Compared to control group, the expression of mTORC1 and HIF-1α in Skm-1 cells treated with differentconcentrations of BIIB021 was significantly decreased（P〈0.05）, and the expression was the lowest in the group of 400 nmol/L.Conclusion BIIB021 inhibits proliferation of MDS Skm-1 cells through down-regulating the expression of mTORC1 and HIF-1α.

关 键 词：HSP-90 骨髓增生异常综合征 mTORC1 HIF-1Α 

分 类 号：R551.3[医药卫生—血液循环系统疾病]