坎离颗粒干预对自发性高血压大鼠和腹主动脉缩窄大鼠的降压疗效  

Effect of Kanli Granule on hypertension in SHR and abdominal aorta constriction rats

作　　者：谭艳[1] 鲁成[1,2] 蒋梅先[1] 刘永明[1] 

机构地区：[1]上海中医药大学附属曙光医院心内科,上海201203 [2]上海中医药大学附属第七人民医院心内科,上海200137

出　　处：《中国医药导报》2017年第12期8-11,16,共5页China Medical Herald

基　　金：国家自然科学基金项目(81102671)

摘　　要：目的观察坎离颗粒干预对自发性高血压大鼠(SHR)及腹主动脉缩窄大鼠的降压疗效。方法 (1)SPF级10周龄雄性SHR大鼠30只,按随机数字表法随机分为对照组(蒸馏水10 m L/kg)、坎离组(坎离颗粒混悬液6.75 g/kg)、缬沙坦组(缬沙坦混悬液7.2 mg/kg),每组10只。12周龄时开始药物干预,共干预12周。(2)SPF级10周龄雄性Wistar大鼠40只,11周龄时,按随机数字表法随机选取10只为假手术组(蒸馏水10 m L/kg),余30只腹主动脉缩窄术后,再次随机分为模型组(蒸馏水10 m L/kg)、坎离组(坎离颗粒混悬液6.75 g/kg)、缬沙坦组(缬沙坦混悬液7.2 mg/kg),每组10只。12周龄时(造模后1周)开始药物干预,共干预12周。分别于治疗前、后4、8、12周测量大鼠血压,各测3次取平均值。结果 SHR血压:(1)与对照组比较,在治疗后第8周、第12周时坎离组血压均明显降低(P<0.01),缬沙坦组治疗第4周、第8周、第12周血压明显降低(P<0.01);与缬沙坦组比较,坎离组各时点血压差异无统计学意义(P>0.05)。(2)与本组治疗前比较,对照组和坎离组在治疗后第4周、第8周、第12周血压均明显增高(P<0.01);缬沙坦组在治疗后8、12周血压均明显增高(P<0.01)。腹主动脉缩窄大鼠血压:(1)造模1、4、8、12周后,模型组明显升高(P<0.01);与模型组比较,坎离组治疗后第8周、第12周血压均明显降低(P<0.01),缬沙坦组治疗后第4、8、12周血压均明显降低(P<0.01);与缬沙坦组比较,治疗后第8周、第12周时,中药组血压较高(P<0.05)。(2)与本组治疗前相比,假手术组血压于治疗后8、12周血压明显增高(P<0.05);模型组血压于治疗后8周、12周血压明显增高(P<0.05);坎离组血压未见明显变化,差异无统计学意义(P>0.05);缬沙坦组血压于治疗后4、8周血压有降低趋势,但差异无统计学意义(P>0.05),至治疗后12周时血压明显降低(P<0.05)。结论坎离颗粒可以显著降低SHR及腹主动脉缩窄大鼠的血压。Objective To investigate the antihypertensive efficacy of Kanli Granule on spontaneously hypertensive rats （SHR）and abdominal aorta constriction rats. Methods By using random number table, thirty SPF male SHRs, ten weeks old, were ramdomly divided into three groups： control group （treated with distilled water 10 mL/kg）, Kanli group （treated with Kanli Granule suspension 6.75 g/kg） and Valsartan group （treated with Valsartan suspension 7.2 mg/kg）. Each group was consisted of ten rats. All the animals were treated for twelve weeks from twelve weeks old. By using random number table, forty SPF male Wistar rats （ten weeks old）, ten were ramdomly received a sham operation, which were treated with distilled water （10 mL/kg） one week later, while thirty with abdominal aorta constriction surgery were ramdomly divided into three groups： model group （treated with distilled water 10 mIJkg）, Kanli group （treated with Kanli Granule suspension 6.75 g/kg） and Valsartan group （treated with valsartan suspension 7.2 mg/kg）. Each group was consisted of ten rats. All the animals were treated for twelve weeks from twelve-week-old （one week after operation as well）. Blood pressure was measured before treatment, and at the fourth, eighth, twelfth week after treatment, and everyone were measured three times to get the mean value eventually. Results The study of SHR：（1）at the eighth and twelfth week after the treatment, compared with the control group, the blood pressure of the Kanli group was significantly reduced （P 〈 0.01）. Compared with the control group, the blood pressure of Valsartan group was reduced at the fourth, eighth and twelfth week after the trentment （P 〈 0.01）. While there was no significant difference between the Kanli group and the Valsartan group （P 〉 0.05）. （2）Compared with the group before treatment, the blood pressure of the control group and the Kanli group were significantly increased at the fourth, eighth and twelfth week after the treatment

关 键 词：坎离颗粒 自发性高血压大鼠 腹主动脉缩窄大鼠 血压 

分 类 号：R28[医药卫生—中药学]