热习服对湿热环境运动刺激大鼠大脑皮质和心肌线粒体的影响  

Effect of heat acclimation on mitochondrion of cerebral cortex and myocardium in rats stimulated by exercise in hygrothermal environment

作　　者：梁涵[1] 李玉祥[1,2] 陈新民[1] 任浩[3] 常文军[1] 史仍飞[2] 马文领[1] 

机构地区：[1]第二军医大学热带医学与公共卫生学系环境卫生学教研室,上海200433 [2]上海体育学院运动科学学院运动生理化学与营养学教研室,上海200438 [3]第二军医大学热带医学与公共卫生学系生物防御教研室,上海市医学生物防护重点实验室,上海200433

出　　处：《第二军医大学学报》2017年第4期469-475,共7页Academic Journal of Second Military Medical University

基　　金：军队后勤科研重点项目(BWS14J-023);军队“十二五”重大专项(AWS12J002-05-02);军队医学技术青年培育项目(15QNP055)~~

摘　　要：目的探究热习服是否能够对湿热环境下进行中高强度运动大鼠的大脑皮质和心肌线粒体结构与功能起保护作用,并探讨其保护机制。方法 40只SD大鼠通过负重力竭实验剔除游泳时间过短和过长的大鼠各4只,剩余32只大鼠随机分成常温自由活动(RA)组、常温运动(RE)组、高温自由活动(HA)组和高温运动(HE)组,每组8只。RA和HA组大鼠分别置于常温[(24±1)℃]和高温[(32±1)℃]条件下自由活动2h/d;RE和HE组大鼠分别置于常温[(24±1)℃]和高温[(32±1)℃]条件下的饲养笼内自由活动1h/d后,在动物跑步机上匀速跑步1h/d(坡度为0°,速度为14m/min);连续训练14d建立热习服大鼠模型。建模后第3天开始,各组大鼠在湿热环境下中高强度运动30min。测量并记录湿热环境下中高强度运动前、后大鼠体质量丢失率、肛温,采用放射免疫法测量血浆去甲肾上腺素(NE)、血管加压素(AVP)和皮质醇(Cort)含量,透射电镜观察运动后大鼠大脑皮质和心肌线粒体的超微结构,ELISA法检测ATP合成酶和活性氧(ROS)含量,总抗氧化能力测试(ABTS)法检测总抗氧化能力。结果 HE组大鼠在湿热环境下中高强度运动后体质量丢失率高于RA组(P<0.01);HE组大鼠运动前、后肛温以及肛温升高幅度均低于RA、RE和HA组(P<0.05,P<0.01)。HE组大鼠运动后血浆中Cort含量低于RA、RE和HA组(P<0.05,P<0.01),大脑皮质和心肌线粒体中ATP合成酶和组织中总抗氧化能力高于RA、RE和HA组(P<0.01),ROS含量低于RA、RE和HA组(P<0.01)。HE组大鼠大脑皮质中线粒体和突触结构以及心肌中线粒体、Z线和横小管的结构优于RA、RE和HA组。结论热习服训练能够增强线粒体氧化呼吸功能、降低细胞的氧化应激水平和血浆中Cort含量,从而保护湿热环境下中高强度运动后大鼠的大脑皮质神经元和心肌细胞线粒体结构。Objective To explore whether heat acclimation can protect the structure and function of mitochondrion of cerebral cortex and myocardium in rats with moderate and high intensity exercise in hygrothermal environment, and to explore its protective mechanism. Methods Forty SD rats underwent swimming experiment bearing weight and 8 rats were eliminated due to too short-swim or long-swim time. The rest 32 rats were randomly divided into four groups （n=8）; room-temperature activity （RA） group, room-temperature exercise （RE） group, high-temperature activity （HA） group and high-temperature exercise （HE） group. Rats in RA and HA groups were placed at room temperature （[24± 17]℃） and high temperature （[32± 1] ℃） to have a free activity for 2 h/d. Rats in RE and HE groups were placed at room temperature （[24± 1]℃） and high temperature （[32± 1]℃） to have a free activity for 1 h/d and then were allowed to run at a constant speed on the animal treadmill for 1 h/d （slope： 0°, speed： 14 m/min）. After 14 days the heat acclimation rat models were created. Rats in all groups underwent moderate and high intensity exercise in hygrothermal environment for 30 min on the 3rd day. The body weight loss rate and rectal temperatures of rats were measured before and after high and medium intensity exercise in hygrothermal environment. The contents of plasma norepinephrine （NE）, arginine vasopressin （AVP） and cortisol （Cort） in rats were detected by radioimmuaoassay. The ultrastructure of the mitochondria of cerebral cortex and myocardium was observed by transmission electron microscopy. The contents of ATP synthase and reactive oxygen species （RIDS） were detected by ELISA method and the total antioxidative capacity was measured by ABTS method. Results the ascending range of recta] temperatures of rats in HE group HA groups （P 〈 0. 05, P 〈 0. 01）. After stimulation with The rectal temperatures before and after stimulation and were significantly lower than those in

关 键 词：热习服 线粒体 大脑皮质 运动 氧化性应激 

分 类 号：R821.45[医药卫生—临床医学]