在云母晶面上应用“自下而上”和“自上而下”两种途径制造胶原蛋白纳米线阵列  

Creating Collagen Nanowire Arrays by ‘Bottom-Up' and ‘Top-Down' Approaches on Mica Lattice Planes

作　　者：李力[1] 张磊[1] 杨德良[1] 孙铭[1] 曾凡喜[1] 顾文华[1] 

机构地区：[1]南京理工大学电子工程与光电技术学院,南京210094

出　　处：《无机化学学报》2017年第5期745-752,共8页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.51303082);中央高校基本科研业务费专项资金(No.30915011316);江苏省"高层次创新创业人才引进计划"资助项目

摘　　要：以云母晶体的(001)晶面为基质,以天然Ⅰ型鼠尾胶原蛋白单体溶液为原料,分别考察了蛋白单体受基质规导进行"自下而上"的自组装过程,以及原子力显微镜探针对吸附在基质表面的蛋白膜层进行"自上而下"加工过程:(1)两种制备途径均能加工出结构精确可控的胶原蛋白纳米线阵列;(2)"自下而上"制备途径利用了蛋白单体和基质晶体在界面上互相识别并规范的"反相生物矿化"原理;(3)"自上而下"的加工利用了原子力显微镜接触模式下探针的"分子扫帚"机理。By using the mica （001） lattice plane as the substrate and rat tail type I collagen monomers as adsorbates, both ＇bottom-up＇ self-assembly of protein monomers guided by the substrate and ＇top-down＇ manipulation of protein membrane by AFM tip were investigated： （1） both preparation approaches can fabricate collagen nanowire arrays with precisely controllable structures; （2） the ＇bottom-up＇ approach was due to the ＇reverse-phase biomineralization＇ mechanism arising from the cooperative recognition and regulation between the protein and substrate at the interface; （3） the ＇top-down＇ manipulation was based on the ＇molecular broom＇ mechanism of the AFM tip in the Contact Mode.

关 键 词：胶原蛋白 自上而下 自下而上 纳米线 介观尺度 

分 类 号：O629.73[理学—有机化学]