一种同时分辨识别Zn^(2+)和F^-离子的双功能探针（英文）  

A Readily Accessible Difunctional Probe: Simultaneous Recognition of Cation Zn^(2+) and Anion F^- via Distinguishable Wavelengths

作　　者：曾莉[1] 赵江林[2] 牟兰[1] 曾晞[1] 卫钢 Redshaw Carl 金宗文 

机构地区：[1]贵州大学贵州省大环与超分子化学重点实验室,贵阳550025 [2]中国科学院深圳先进技术研究院生物医学与健康工程研究所微纳系统与仿生医学研究中心,深圳518055 [3]澳大利亚联邦科学与工业研究组织 [4]英国赫尔大学化学系

出　　处：《无机化学学报》2017年第5期769-778,共10页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.21165006);教育部"春晖计划"(No.Z2015007,Z2016008);深圳市基础研究项目(No.JCYJ20140610151856726);深圳市技术攻关项目(No.JSGG20141118113131546);中国博士后自然基金(No.2015M582439)资助

摘　　要：由8-甲酰基-7-羟基香豆素与碳酰肼经一步缩合反应可制得探针1。研究发现,探针1对Zn^(2+)和F-离子均呈现荧光增强和比率比色的高灵敏和高选择性响应,检出限低至10-8 mol·L^(-1)。通过光谱、ITC、1H NMR滴定及质谱分析,详细地研究了探针与离子形成的配合物性质。在不同的介质中,探针不仅同时表现出对金属阳离子Zn^(2+)和对阴离子F-的识别,而且吸收和发射波长均有显著的差异:1-Zn^(2+)配合物的最大吸收波长为360 nm,而1-F-配合物为400 nm;1-Zn^(2+)配合物的荧光激发和发射波长分别为360和454 nm,而1-F-配合物分别为400和475 nm。此外,探针1还能应用于活体PC3细胞中Zn^(2+)的荧光成像。The probe 1 was readily prepared via condensation of 8-formyl-7-hydroxy-coumarin and carbonic dihydrazide in a one-step procedure. Probe 1 exhibited high sensitivity and selectivity towards Zn2＋ and F- through a ＂turn-on＂ fluorescence response and/or ratiometric eolorimetric response with low detection limits of the order of 10-8 mol.L-1. The complex behaviour was fully investigated by spectral titration, isothermal titration calorimetry, 1H NMR spectroscopic titration and mass spectrometry. Interestingly, probe 1 not only recognizes the cation Zn2＋ and the anion F-, but can also distinguish between these two ions via the max wavelength in their UV- Vis spectra （360 nm for 1-Zn2＋ vs 400 nm for I-F- complex） or their fluorescent spectra （Aex, kern=360, 454 nm for 1-Zn2＋ vs An, Aem=400, 475 nm for I-F- complex） due to their differing red-shifts. Additionally, probe 1 has been further explored in the detection of Zn2＋ in living cells.

关 键 词：8-甲酰基-7-羟基香豆素 Zn2+ F- 分辨波长 细胞成像 

分 类 号：O657[理学—分析化学]