自发性糖尿病长爪沙鼠环氧化酶(COX)-2在三种组织中的表达  

Analysis on Cyclooxygenase 2 Expression in Different Tissues from Spontaneous Diabetic Mongolian Gerbils

作　　者：王菲菲[1] 龚菁菁[1] 霍学云[1] 路静[1] 郭萌[1] 刘欣[1] 李长龙[1] 杜小燕[1] 陈振文[1] 吕建祎[1] WANG Fei-fei GONG Jing-jing HUO Xue-yun LU-jing GUO-Meng LIU-Xin LI Chang-long DU Xiao-yan CHEN Zhen-wen LV Jian-yi(School of Basic Medical Science, Capital Medical University, Beijing 100069, China)

机构地区：[1]首都医科大学基础医学院,北京100069

出　　处：《实验动物与比较医学》2017年第2期118-122,共5页Laboratory Animal and Comparative Medicine

基　　金：国家科技支撑计划(No.2015BAI09B01);国家自然科学基金(Nos.31272393 and 31402027);北京市自然科学基金(No.7141002)

摘　　要：目的研究环氧化酶(COX)-2基因在糖尿病长爪沙鼠3种组织中的表达水平,进一步探索其与长爪沙鼠糖尿病发生和发展的关系。方法选取糖尿病长爪沙鼠和正常沙鼠各6只,分别采集动物的骨骼肌、肝脏和肾脏组织,用实时PCR和Western blotting检测COX-2基因在各组织中mRNA和蛋白表达水平。结果实时PCR的结果表明,COX-2基因的mRNA水平在糖尿病组动物的骨骼肌组织中表达与对照组无明显差异,而肝脏和肾脏组织中有表达升高趋势。Western blotting结果显示,糖尿病组COX-2的蛋白水平在肝脏和肾脏组织中存在表达升高趋势,且肾脏具有统计学意义。结论 COX-2在糖尿病长爪沙鼠肝脏和肾脏组织中表达升高,在肾脏组织尤其明显,说明COX-2对长爪沙鼠糖尿病的影响可能发生在肝脏和肾脏组织中。Objective To analyze Cyclooxygenase 2（COX-2） expression level in different tissues of diabetic gerbils and to explore the relationship of diabetes occurrence and development in Mongolian gerbils. Methods Different tissues including skeletal muscle, liver and kidney were collected from 6 diabetic gerbils and 6 control animals respectively. COX-2 mRNA and protein expression level in different tissues were separately detected by Real-time PCR and Western blotting. Results The Real-time PCR showed that compared with control animal, the mRNA expression of COX-2 in skeletal muscle had no obvious difference whereas it exhibited uptrend in liver and kidney. As the results of Western blotting, the difference of COX-2 in skeletal muscle was almost undetectable in diabetes group. The relative expression level in diabetic group exhibited uptrend compared with control in liver and kidney. And there was statistical significance in the kidney. Conclusions COX-2 was high expressed in liver and more remarkably in kidney of diabetic gerbils. It may illustrate that the effect of COX-2 mainly occurred in liver and kidney of diabetic gerbils.

关 键 词：长爪沙鼠II型糖尿病COX-2 实时PCRWestern BLOTTING 

分 类 号：R587.1[医药卫生—内分泌]