黄连解毒汤对大鼠亚慢性毒性试验研究  

Subchronic Toxicity of Coptidis Toxin Resolving Decoction in Rats

作　　者：刘芳[1,2] 王立红[1,2] 董惠峰 赵晶晶[1,2] 张俊霞[2] 刘鼎阔[3,1,2] 

机构地区：[1]天津市饲用微生物制剂企业重点实验室,天津300383 [2]鼎正动物药业(天津)有限公司,天津300383 [3]中国农业大学动物医学学院,北京100094

出　　处：《天津农学院学报》2017年第1期23-27,共5页Journal of Tianjin Agricultural University

基　　金：天津市科技支撑计划项目"中草药在水产健康养殖中的应用与开发"(13ZCZDNC00700)

摘　　要：研究黄连解毒汤连续灌服情况下对于SD大鼠的亚慢性毒性作用,揭示其临床安全性。SD大鼠120只,雌雄各半,随机分为对照组和黄连解毒汤20、10、5 m L/kg剂量组,每组30只,连续给药6周,以SD大鼠的体重、血液学、血液生化学、组织病理学检查作为检查指标,全面评价连续灌服对大鼠的毒性作用。连续给药42 d后,与对照组相比,黄连解毒汤试验组大鼠的血常规及各项血液生化检测指标结果相近(P>0.05)。组织病理学切片结果显示,黄连解毒汤20 m L/kg组大鼠肝脏和肾脏出现了轻微损伤,10 m L/kg和5 m L/kg剂量组SD大鼠的的脾、肺、肝、肾、胃等组织亦均未发现异常的组织病理学变化。黄连解毒汤在10 m L/kg和5 m L/kg剂量下连续灌服情况下对SD大鼠无不良影响。The subchronic toxicity of Coptidis Toxin Resolving Decoction in SD rats is researched by continuous perfusion in order to expose its clinical innocuity. 120 SD rats （males and females） were randomly devivided into control group and Coptidis Toxin Resolving Decoction in 3 different dosage groups （20, 10, and 5 mL/kg, 30 in each group）. The SD rats were administered with Coptidis Toxin Resolving Decoction by continuous perfusion for 6 weeks. The body weight, hematology, blood biochemistry and histopathology examination were measured for the overall toxicity assessment to rats. After 42 days, contrasted with the control group, the test group rats blood routine examination index and blood index had an approximately result （P〉0.05） . The result ofhistopathological slices showed that the liver and kidney of Coptidis Toxin Resolving Decoction 20 mL/kg group rats appeared slight damage, while the spleen, lung, liver, kidney and stomach of Coptidis Toxin Resolving Decoction 10 mL/kg group and 5 mL/kg group does not find abnormal histopathologic change. Conclusion With a dose of 10 mL/kg and 5 mL/kg Coptidis Toxin Resolving Decoction continuous perfusion, there is not harmful effect on SD rats.

关 键 词：黄连解毒汤 大鼠 亚慢性毒性 

分 类 号：S854.5[农业科学—临床兽医学]