雷帕霉素通过促进自噬提高小鼠iPSCs诱导效率  

Rapamycin increased inducible efficiency of iPSCs through activating autophagy

作　　者：梁浩然[1,2] 曹安[1] 韩建永[2] 曹素英[1] 

机构地区：[1]北京农学院动物科学技术学院,北京102206 [2]中国农业大学生物学院,北京100193

出　　处：《北京农学院学报》2017年第2期60-64,共5页Journal of Beijing University of Agriculture

基　　金：国家自然基金青年项目(31601941);北京市自然基金(6152004);北京市教委项目(PXM2016_014207_000008);北京市大学生科研训练项目(5046516564/003);2015科研水平提高经费-高级别科研项目培育(1086716274)

摘　　要：【目的】雷帕霉素(Rapamycin)可以激活和促进细胞的自噬过程。探明在体外培养系统中添加雷帕霉素,检测是否可以通过促进自噬过程提高iPSCs诱导效率。【方法】在iPSCs诱导过程的1~3d和4~6d分别加入雷帕霉素,检测自噬现象,同时对iPSCs诱导过程中多能性基因表达进行检测,统计原代iPSCs克隆数等,评价雷帕霉素对iPSCs重编程的影响;在iPSCs的培养过程中分别加入50nM/L和100nM/L的雷帕霉素处理。【结果】使用浓度为50nM/L和100nM/L的雷帕霉素处理,可以提高iPSCs的诱导效率。50nM/L浓度处理,重编程效率较高【结论】雷帕霉素在iPSCs重编程过程中可以促进自噬,提高Nanog表达水平,抑制p53表达,进而提高iPSCs诱导效率。[Objective]Rapamycin could activate and accelerate autophagy process of cell. The aim of this experiment was to in- vestigate if rapamycin could increase the inducible efficiency of iPSCs through adding rapamycin in in-vitro cell culture system. [Methods] The autophagy was detected by adding rapamycin to culture system in the day 1-3 and day 4-6 during the process of iPSCs induction.At the same time, the expression level of pluripotent genes were measured. The amount of primary iPSCs colo- nies were calculated and the effect of Rapamycin on cell reprogamming was evaluated. 50 nM/L and 100 nM/L rapamycin were used in the process of culture.[Results]The result showed that adding 50 nM/L and 100 nM/L rapamycin in the culture system could increase the iPSc induction efficiency. And 50 nM/L rapamycin demonstrated a better reprogramming efficiency.[Conclu- sion]In conclusion, rapamycin could improve the inducible efficiency of iPSCs by means of stimulating autophagy, increasing the level of Nanog and inhibiting p53 expressions.

关 键 词：诱导多能性干细胞 自噬 重编程 

分 类 号：S188[农业科学—农业基础科学]