四逆散有效组分对慢性情绪应激所致大鼠睡眠障碍的改善作用研究  

Active Compounds of Sinisan for Improving Sleep Disorder of Rats with Chronic Emotional Stress

作　　者：叶晓楠[1] 陈光[1] 卞宏生[1] 李廷利[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药学报》2017年第2期6-10,共5页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金面上项目(87214114);黑龙江中医药大学博士创新基金(2015bs08)

摘　　要：目的:基于空瓶刺激诱发慢性情绪应激所致大鼠睡眠障碍模型来研究四逆散有效组分是通过调整负性情绪来实现其改善睡眠的作用。方法:SD大鼠24只,随机分为空白对照组、模型组、阳性药物对照地西泮组(1 mg/kg)、四逆散有效组分组(450 mg/kg),每组6只。以空瓶刺激法建立情绪应激模型。在模型复制的第2周开始灌胃给药,共灌胃7 d。观察四逆散有效组分对慢性情绪应激大鼠的攻击行为、睡眠时间、睡眠-觉醒节律,全脑TNF-α含量的影响。结果:与空白对照组比较,模型组大鼠的攻击行为显著增多(P<0.01),全天睡眠时间显著减少(P<0.05),全脑TNF-α水平显著降低(P<0.01);与模型组比较,四逆散有效组分可使攻击行为显著减少(P<0.05),全天睡眠时间显著增多(P<0.05),全脑TNF-α水平显著增高(P<0.05)。结论:四逆散有效组分可通过改善负性情绪来实现其改善睡眠的作用,对全脑TNF-α水平的调节可能是其作用的机制之一。Objective：To study the effect of Sinisan on sleep disorder of rats with chronic emotional stress induced by the empty bottle stimulation.Methods：Twenty-four male SD rats were randomly divided into four groups（ six rats in each group）,empty bottle stimulation group,blank control group,diazepam positive control group diazepam（ 1 mg/kg）,Sinisan＇s active compounds treatment group（ 450 mg/kg）.The chronic emotional stress rats（ model） group was established by empty bottle stimulation for 2 weeks.On the 2nd week after empty bottle stimulation,the treatment groups were treated by the corresponding medicine once a day for 7 days by lavage after the model establishment.Observe the effect of Sinisan ＇s active compounds on aggressive behaviors,sleep time,sleep-wake rhythm,and the whole brain TNF-α level.Results：Compared with those in the control group,the aggressive behaviors of rats in the model group was significantly increased（ P〈0.01）,and it was significantly reduced in sleep time（ P〈0.05） and the whole brain TNF-α level（ P〈0.01）.Compared with empty bottle stimulation group,Sinisan treatment group showed a significant reduction in aggressive behaviors（ P〈0.05）,while the sleep time and the whole brain TNF-α level were significantly increased（ P〈0.05）.Conclusion：The active compounds of Sinisan can improve sleep disorder by way of improving the negative emotion and one of its mechanisms is through TNF-α regulation for the brain.

关 键 词：空瓶刺激 应激 负性情绪 睡眠障碍 细胞因子 

分 类 号：R285.5[医药卫生—中药学]