归芪多糖对衰老大鼠肾组织氧化损伤作用的研究  

Effect of Guiqi Polysaccharide on Oxidative Damage of Kidney Tissue in Aging Rats

作　　者：蒲秀瑛[1] 李海兵[1] 李晓玥[1] 马小龙[1] 刘璐[1] 任菁[1] 

机构地区：[1]兰州理工大学生命科学与工程学院,甘肃省中藏药筛选评价及深加工重点实验室,甘肃兰州730050

出　　处：《中医药学报》2017年第2期15-18,共4页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金项目(81260070)

摘　　要：目的:研究归芪多糖对D-半乳糖所致衰老大鼠肾组织氧化损伤的影响。方法:通过水提醇沉法从一定比例的当归和黄芪中提取出归芪多糖,并用苯酚-硫酸法进行多糖含量测定;除正常对照组外其余各组均采用D-半乳糖(0.15 g/kg/d)皮下注射建立大鼠衰老模型;以不同剂量的归芪多糖(2.0g/kg和0.5 g/kg)给大鼠连续灌胃42 d后,测定其肾组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活力、谷胱甘肽(GSH)含量、丙二醛(MDA)含量和蛋白质羰基含量。结果:归芪多糖可增强衰老大鼠肾组织中的SOD、GSH-PX活力和GSH含量,显著降低MDA和蛋白羰基含量。结论:归芪多糖可保护D-半乳糖所致衰老大鼠肾组织的氧化损伤,从而起到保护肾脏衰老的作用。Objective：To study the effect of Guiqi polysaccharide on oxidative damage of kidney tissue in D-galactose-induced aging rats.Methods：We extracted polysaccharide from a percentage of angelica and astragalus membranaceus through the water extraction and alcohol precipitation method,and determined the content of polysaccharide with phenol sulfuric acid method.D-galactose was subcutaneous injected at a dose of 0.15 g/kg body weight every day except the normal control group.Rats of the low-and high-dose Guiqi polysaccharide（ 2.0 g/kg and 0.5 g/kg） were orally administrated daily for 42 days,respectively.Then superoxide dismutase（ SOD） activity,the content of glutathione peroxidase（ GSH-PX）,glutathione（ GSH）,malondialdehyde（ MDA） content,protein carbonyl of kidney were determined,respectively.Results：Guiqi polysaccharides could enhance SOD,GSH-PX,GSH enzyme activity in renal tissue of aging rats,and it could significantly decrease the content of MDA and protein carbonyl.Conclusion：Guiqi polysaccharide can reduce free radical damage to the kidney of aging rats and protect the normal kidney from senescence.

关 键 词：归芪多糖 衰老 SOD GSH-PX GSH MDA 蛋白羰基 

分 类 号：R285.5[医药卫生—中药学]