稳定同位素稀释液相色谱质谱法在医药研究中的应用  

Application of stable isotope dilution liquid chromatography-mass spectrometry in medical research

作　　者：谷雨龙[1] 朴香兰[1] 

机构地区：[1]中央民族大学中国少数民族传统医学研究院,北京100081

出　　处：《国际药学研究杂志》2017年第4期326-331,共6页Journal of International Pharmaceutical Research

基　　金：国家自然科学基金资助项目(81603278);教育部"长江学者和创新团队发展计划"资助项目(IRT_13R63);中央高校基本科研业务费

摘　　要：稳定同位素稀释质谱法(ID-MS)是指以稳定同位素标记化合物为内标,测定非标记化合物含量的一种定量方法。稳定同位素稀释液相色谱质谱法(ID-LC-MS)具有检测灵敏度高、不需要对待测物定量分离、有效消除信号所受到的基质效应干扰等优点。该法在生物标志物、药物及其代谢物定量分析研究中得到广泛应用。本文简要介绍ID-LC-MS的机制和特点,并对其在医药研究领域中的应用进行概述。Stable isotope dilution mass spectrometry＇ （ID-MS） is a quantitative method for determining the content of unla- beled compounds with stable isotope labeled compounds as internal standard. Stable isotope dilution liquid chromatography-mass spectrometry （ID-LC-MS） has the advantages of high sensitivity, no quantitative separation of the analytes, and effective elimination of the biological matrices. The method has been widely used in quantitative analysis of biomarkers, drugs and their metabolites. In this paper, the mechanism and characteristics of ID-LC-MS are briefly introduced, and its medical applications are summarized.

关 键 词：稳定同位素稀释液相色谱质谱法 药物 生物标志物 

分 类 号：O657.63[理学—分析化学] R-05[理学—化学]