工频磁场对P38丝裂原活化的蛋白激酶磷酸化的诱导作用  

Exposure to power-frequency magnetic fields can induce activation of P38 mitogen-activated protein kinase

作　　者：孙文均[1] 余应年[2] 姜槐[1] 付一提[1] 鲁德强[1] 

机构地区：[1]浙江大学医学院微波研究室,杭州310031 [2]浙江大学医学院病理生理教研室,杭州310031

出　　处：《中华劳动卫生职业病杂志》2002年第4期252-255,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金重点资助项目 (3 963 0 10 0 )

摘　　要：目的　研究 5 0Hz工频磁场对细胞内P38丝裂原活化的蛋白激酶 (P38mitogen activatedproteinkinase ,P38MAPK)信号转导途径的影响 ,阐明工频磁场生物效应相关的细胞信号转导机制。方法　中国仓鼠肺成纤维细胞 (CHL)分别经 0 .1mT及 0 .4mT 2个强度的工频磁场辐照不同时间后 ,提取细胞蛋白质 ,以Western印迹技术 ,测定并比较细胞内P38MAPK及其上游激酶MKK3/MKK6(MAPKkinase 3/ 6 )磷酸化 (活化 )的变化。结果　 0 .1mT的工频磁场在 2 4h内不能诱导P38MAPK磷酸化 ,而 0 .4mT处理 10min即开始诱导P38MAPK磷酸化 ,15min后P38MAPK磷酸化恢复至对照状态。但 2个强度都不能诱导上游激酶MKK3/MKK6的磷酸化 (活化 )。结论　 5 0Hz工频磁场可以短暂诱导P38MAPK磷酸化 (活化 ) 。Objective To study the effects of 50 Hz power frequency magnetic fields on signal transduction pathway of P38 mitogen activated protein kinase(P38 MAPK),and explore the cellular signal transduction mechanism of the biological effects induced by power frequency magnetic fields. Methods Chinese hamster lung(CHL) cell line was exposed to power frequency magnetic fields with two intensities(0.1 and 0.4 mT) for different exposure durations.The cytoplasmic protein was extracted.The phosphorylated(activated) and non phosphorylated P38 MAPK and MKK3/MKK6 were measured by Western blotting analysis with their specific corresponding antibodies. Results Power frequency magnetic fields at 0.4 mT for 10 min could transitorily induce the activation of P38 MAPK and after 15 min the phosphorylation of P38 MAPK restored to control level,while 0.1 mT power frequency magnetic fields could not induce the activation of P38 MAPK within 24 h.However,both 0.1 mT and 0.4 mT power frequency magnetic fields could not phosphorylate(activate) the MKK3/MKK6,which is a general upstream kinase of P38 MAPK. Conclusion Power frequency magnetic fields could transitorily activate the P38 MAPK,but not MKK3/MKK6.The activation mechanism of P38 MAPK needs to be further identified.

关 键 词：工频磁场 P38丝裂原活化的蛋白激酶 P38 MAPK 信号转导 磷酸化 

分 类 号：X591[环境科学与工程—环境工程]