极低频电磁场及与铅联合作用对小鼠抗氧化系统的影响  

Effects of extremely low frequency electromagnetic field and its combination with lead on the antioxidant system in mouse

作　　者：刘贇[1] 翁恩琪[1] 张颖[1] 洪蓉[1] 

机构地区：[1]华东师范大学环境科学系环境生理与毒理研究室,上海200062

出　　处：《中华劳动卫生职业病杂志》2002年第4期263-265,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的　研究极低频电磁场及其与铅的联合作用对小鼠脑和肝脏抗氧化系统的影响。方法　将小鼠暴露于 5 0Hz、0 .2mT或 6 .0mT的电磁场中 ,持续 2周 ,同时染铅 (5 0mg/kg) ,观察小鼠脑和肝脏氧化、抗氧化系统和细胞膜流动性的变化。结果　电磁场暴露下 ,脑和肝组织丙二醛 (MDA)含量分别为 (1.35± 0 .0 9)、(6 .15± 0 .2 8)nmol/mgpro(0 .2mT)和 (3.98± 0 .10 )、(6 .5 0± 0 .79)nmol/mgpro(6 .0mT) ,较对照组 [分别为 (1.33± 0 .12 )、(3.95± 0 .2 1)nmol/mgpro]高 ,差异有显著性 (P<0 .0 5 ) ;总抗氧化能力 (T AOC)分别为 (3.99± 0 .39)、(1.92± 0 .32 )U/mgpro(0 .2mT)和 (3.12±0 .37)、(1.5 7± 0 .14 )U/mgpro(6 .0mT) ,较对照组 [分别为 (4.39± 0 .4 8)、(2 .4 5± 0 .2 1)U/mgpro]明显升高 ;肝谷胱甘肽 (GSH)含量也较对照组有所下降。脑细胞和肝细胞膜流动性分别为 1.2 2 4± 0 .190、1.894± 0 .0 76 (0 .2mT)和 1.15 9± 0 .179、1.5 16± 0 .2 0 4 (6 .0mT) ,较对照组 (分别为 1.396± 0 .0 4 0、2 .899± 0 .5 5 2 )下降。电磁场暴露 (6 .0mT)合并染铅 ,与单独电磁场暴露组相比 ,脑和肝脏组织MDA含量分别升高为 (8.4 0± 0 .72 )、(12 .88± 1.0 9)nmol/mgpro ,GSH含量分别升高为Objective To study the effects of extremely low frequency electromagnetic field(ELF EMF) and its combination with lead on the antioxidant system in mouse brain and liver tissues. Method Mice were exposed to a 50 Hz sinusoidal 0.2 mT or 6.0 mT EMF for 2 weeks.At the same time,some groups were exposed to lead(50 mg/kg).After the exposure,the antioxidant system and cell membrane fluidity in brain and liver were measured. Results Malondiadehyde(MDA) content in brain and liver increased from the control levels of (1.33± 0.12) and (3.95±0.21)nmol/mg pro to (1.35±0.09) and (6.15±0.28)nmol/mg pro respectively following 0.2 mT exposure,and to (3.98±0.10) and (6.50±0.79)nmol/mg pro respectively following 6.0 mT exposure.Total antioxidant capability(T AOC) in brain and liver decreased from the control levels of (4.39±0.48) and (2.45 ±0.21)U/mg pro to (3.99±0.39) and (1.92±0.32)U/mg pro respectively following 0.2 mT,and to (3.12 ±0.37) and (1.57±0.14)U/mg pro respectively following 6.0 mT.GSH content decreased only in liver tissue from the control level of (194.60±20.93)mg/g pro to (189.24±5.61)mg/g pro(0.2 mT) and (153.04±1.18)mg/g pro(6.0 mT).Cellular membrane fluidity decreased from the control levels of (1.396±0.040) and (2.899±0.552) to (1.224±0.190) and (1.894±0.076 1)(0.2 mT),(1.159±0.179) and (1.516± 0.204) (6.0 mT) respectively.Compared with single EMF exposure(6.0 mT),EMF combined with lead exposure induced remarkable increase in MDA,GSH content and T AOC and decrease in cell membrane fluidity both in the brain and liver,and increase in SOD activity only in liver. Conclusion ELF EMF might alter the metabolism of free radicals,decrease anti oxidant capability and enhance lipid peroxidation.The combination of EMF with lead showed synergic effects on lipid peroxidation.

关 键 词：铅 极低频电磁场 脂质过氧化 抗氧化系统 膜流动性 生物学效应 

分 类 号：X591[环境科学与工程—环境工程]