GC-1200γ计数器在^(125)I体外细胞摄取实验中测量问题分析  

Investigation on measurement problems of GC-1200γ counter in experiment of ^(125)I in vitro cellular uptake

作　　者：武红玉[1] 陈飞[2] 陈永伟[1] 邹美芬[3] 徐栋[3] 钦晓峰[3] 徐希杰[3] 

机构地区：[1]江苏省原子医学研究所/江原医院检验科,江苏无锡214063 [2]无锡市人民医院核医学科,江苏无锡214043 [3]江苏省原子医学研究所卫生部核医学重点实验室江苏省分子核医学重点实验室,江苏无锡214063

出　　处：《国际检验医学杂志》2017年第9期1153-1154,共2页International Journal of Laboratory Medicine

基　　金：国家自然科学基金青年科学基金项目(21401084)

摘　　要：目的探讨国产GC-1200γ放免计数器在^(125)I-FSH体外细胞摄取实验中可能会影响测量结果的几个相关问题。方法样品进入测量室前先做本底测量;通过变换空白放免管、低计数管、高计数管在同一测量架的不同位置及在待测区的不同位置所测得的CPM进行统计比较。结果在高计数单个样品达1.9×10~6 CPM时对相邻位低计数管计数的影响为7%;对相邻探测器中低计数管计数的影响为7.33%;将所有样本由高到低排列并将高计数样品架放置于紧贴探测器的待测位置,对低计数管的计数影响为5.33%。结论国产GC-1200γ放免计数器适用于^(125)I核素标记的体外细胞摄取实验的测量。Objective To investigate several related problems possibly affecting the measurement results in the experiment of^(125)I-FSH in vitro cells uptake by domestic GC-1200 gamma RIA counter.Methods Before entering the measuring room,the sample was performed the background measurement.CPM measured in the different locations of same measurement frame and the different locations in unmeasured area were performed the statistical comparison.Results In high count,the influence of single sample reaching 1.9 ×10~6 CPM on the adjacent low counting tube count was 7%;its influence on low counting tube count in adjacent detector was 7.33%;all samples were arranged from high to low order and the high count sample holder was placed on the measured location close to the detector,its influence on low counting tube count was 5.33%.Conclusion The domestic GC-1200γRIA counter is suitable for the measurement of the in vitro cell uptake experiment of ^(125)I nuclear labeling.

关 键 词：国产 γ放免计数器 ^125I 体外细胞摄取 测量 

分 类 号：R446.6[医药卫生—诊断学]