子痫前期患者胎盘组织中miR-155及CXCR4的表达及意义  

Expression and significance of miR-155 and CXCR4 in placenta tissues from patients with preeclampsia

作　　者：张展[1,2] 王媛媛[3] 李爱萍[1] 宋婉玉 徐娜[1] 刘慧[1] 

机构地区：[1]郑州大学第三附属医院检验科,郑州450052 [2]河南省商丘医学高等专科学校临床医学系,河南商丘476000 [3]郑州大学第三附属医院妇产科,郑州450052

出　　处：《国际检验医学杂志》2017年第9期1167-1171,共5页International Journal of Laboratory Medicine

基　　金：河南省科技厅开放合作项目(142106000044)

摘　　要：目的探讨子痫前期(PE)患者胎盘组织中微小RNA-155(miR-155)及趋化因子受体4(CXCR4)的表达变化及临床意义。方法选取2015年12月至2016年2月在郑州大学第三附属医院产科以剖宫产术分娩的30例重度子痫前期(sPE)孕妇作为sPE组,同期因社会因素行剖宫产术分娩的30例健康孕妇为健康对照组(N组)。采用实时荧光定量PCR技术(RT-PCR)检测2组孕妇胎盘组织中miR-155及CXCR4mRNA表达水平,并对miR-155含量与CXCR4含量的相关性进行分析。采用免疫组化链霉亲和素-生物素-过氧化物酶复合物(SABC)法,检测同课题组前期构建的胎盘绒毛滋养细胞(VCT)组织芯片(正常对照1组42例,PE组56例)和绒毛外滋养细胞(EVCT)组织芯片(正常对照2组29例,PE组47例)中CXCR4蛋白的表达。结果 (1)2组孕妇的年龄、分娩孕周、孕前体质量指数(BMI)分别比较,差异均无统计学意义(P>0.05)。2组孕妇血压比较,差异有统计学意义(P<0.05)。sPE组新生儿出生体质量明显低于N组,差异有统计学意义(P<0.05)。sPE组孕妇尿蛋白明显高于N组,差异有统计学意义(P<0.05)。(2)胎盘VCT和EVCT组织芯片中:2组孕妇年龄比较,差异无统计学意义(P>0.05);PE组分娩孕周早于N组,PE组孕妇收缩压、舒张压及尿蛋白均高于N组,新生儿出生体质量显著低于N组,差异均具有统计学意义(P<0.05)。(3)sPE组胎盘组织中miR-155mRNA表达水平为1.53±0.92,明显高于对照组的0.87±0.73,差异具有统计学意义(P<0.05)。(4)N组孕妇胎盘组织中CXCR4mRNA含量为1.51±1.85,sPE组为0.54±0.38,两组比较差异有统计学意义(P<0.05)。(5)sPE组孕妇胎盘组织中miR-155含量与CXCR4含量有显著相关性(r=-0.773,P<0.05)。(6)CXCR4蛋白在VCT及EVCT均可见表达;胎盘VCT组织芯片中PE组CXCR4阳性表达率为48.21%(27/56),sPE组阳性表达率为47.92%(23/48),早发型PE组阳性表达率为53.66%(22/41),均显著低于正常对照组的83.33%(35/42),差异均有统计学意义(P<0.05)�Objective To investigate the expression changes and clinical significance of microRNA-155(miR-155)and chemokine receptor 4(CXCR4)in placental tissue from the patients with preeclampsia(PE).Methods Thirty pregnant women with severe PE(sPE)in the Third Affiliated Hospital of Zhengzhou University from December 2015 to February 2016 served as the sPE group,and contemporaneous 30 healthy pregnant women undergoing cesarean section due to the social factors served as the healthy control group(N).The real-time fluorescent quantitative PCR(RT-PCR)was used to detect the expression of miR-155 and CXCR4mRNA in placental tissue and the relationship between miR-155 and CXCR4levels was analyzed.The immunohistochemistry SABC methods were used to detect the expression of CXCR4 protein in villous cytotrophoblast(VCT)tissue microarray(TMA,42 cases in the normal control group 1,56 cases in the PE group)and extravillous cytotrophoblast(EVCT)TMA(29cases in the normal control group 2,47 cases in the PE group)constructed by the same research group.Results(1)There was no statistically significant difference in the age,gestational age and pre-pregnancy body mass index(BMI)between the two groups(P>0.05).The differences of blood pressure between the two groups were statistically significant(P<0.05).The neonatal birthweight in the sPE group was significantly lower than that in the control group with statistically significant differences(P<0.05).The urine protein in the sPE group was significantly higher than that in the control group with statistically significant differences(P<0.05).(2)In the placental VCTand EVCT TMA,the age had no statistical difference between the two groups(P>0.05).The gestational weeks of the PE group were earlier than those in the N group 1,the systolic/diastolic blood pressure and urine protein were higher than those in the N group,the differences all were statistically significant(P<0.05),the neonatal birthweight was significantly lower than that in the N group with statistically significant differences(P<0.05).(3)T

关 键 词：子痫前期 MIR-155 CXCR4 胎盘组织 滋养细胞 

分 类 号：R714.244[医药卫生—妇产科学] R446.1[医药卫生—临床医学]