Na_6PMo_(11)FeO_(40)对小鼠黑色素瘤B16细胞内黑色素合成抑制及抑菌活性研究  

Studies on inhibition of Na_6PMo_(11)FeO_(40) to melanin formation in mouse melanoma B16 Cell and its antibacterial activity

作　　者：邓阳阳[1] 陈丙年[2] 王芳[3] 邢蕊[1] 李莉莉[1] 谢乐芳 王力[1] DENG Yang-yang CHEN Bing-nian WANG Fang XING Rui LI Li-li XIE Le-fang WANG Li(College of Food and Biological Engineering,Jimei University,Xiamen 361021 ,China Yanwu Affiliated Hospital of Zhongshan Hospital, Xiamen University,Xiamen 361005 ,China College of Chemitry and Life Science, Quanzhou Normal College, Quanzhou 362000, China)

机构地区：[1]集美大学食品与生物工程学院,福建厦门361021 [2]厦门大学附属中山医院演武分院(厦门大学医院),福建厦门361005 [3]泉州师范学院化学与生命科学学院,福建泉州362000

出　　处：《分子科学学报》2017年第2期108-113,共6页Journal of Molecular Science

基　　金：国家自然科学基金资助项目(21371072)

摘　　要：研究了过渡金属取代的Kiggen型结构的杂多酸盐Na_6PMo_(11)FeO_(40)对体外培养的小鼠黑色素瘤B16细胞的生物学效应,并分析了其对4种细菌的抗菌活性.结果显示:不同浓度的Na_6PMo_(11)FeO_(40)对B16细胞的细胞形态和数量有不同程度的影响,对B16细胞的增殖率、酪氨酸酶活性以及黑色素合成量的抑制呈现出明显的浓度效应.浓度为50~200μmol/L的Na_6PMo_(11)FeO_(40)能显著抑制B16细胞增殖(P<0.05或P<0.01);浓度为200μmol/L的Na_6PMo_(11)FeO_(40)对酪氨酸酶活性抑制极显著(P<0.01),IC50为166.5μmol/L;Na_6PMo_(11)FeO_(40)对藤黄八叠球菌、金黄色葡萄球菌、枯草芽孢杆菌和大肠杆菌均有抗菌作用,且对球菌的抑制效果优于杆菌.实验结果表明,Na_6PMo_(11)FeO_(40)可作为具有防腐抑菌功能的新型酪氨酸酶抑制剂.The bio-efficacy of Keggen-type Fe-substituted heteropoly salt （Na6PMo11 FeO40） on mouse B16 melanoma cell in vitro was researched,and its antibacterial activity of four species of bacteria was analyzed in this paper. The results showed that the cell morphology and number of B16 cell were changed by different concentrations of Na6 PMo11 FeO40. And the inhibition effect of Na6 PMoll FeO50 on cell proliferation rate, tyrosinase activity and melanin content appears dose-effect relationship. The proliferation of B16 cell was inhibited significantly after treat with 50~200 mol/L of Na6PMo11FeO40 （P〈0.05 or P〈0.01）. The inhibition effect of Na6PMo11 FeO40 on tyrosinase activity is significant （P〈0.01） ,its IC50 is 166.5 mol/L. Na6PMo11FeO40 have an antibacterial effect on the four tested bacteria （Escherichia coli, Bacillus subtilis, Staphylococcus aureus and Sarcina lutea）, and its inhibitory effect on the coccus is better than bacillus. The result indicated that Na6 PMo11 FeO40 can be used as a new tyrosinase inhibitor with antibacterial activity.

关 键 词：杂多酸盐 小鼠黑色素瘤B16细胞 抗菌活性 酪氨酸酶 

分 类 号：O599[理学—应用物理]