硫化氢中毒案件中血液硫离子的测定  

Determination of Sulfide Ion in Blood from Hydrogen Sulfide Poisoning Cases

作　　者：强火生 陈航[2] 沈保华[2] 沈敏[2] 向平[1,2] QIANG Huo-sheng CHEN Hang SHEN Bao-hua SHEN Min XIANG Ping(Pharmacy School of Wenzhou Medical University, Wenzhou 325035, China Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Science, Ministry of Justice, PRC,Shanghai 200063 China)

机构地区：[1]温州医科大学药学院,浙江温州325035 [2]司法部司法鉴定科学技术研究所上海市法医学重点实验室上海市司法鉴定专业技术服务平台,上海200063

出　　处：《法医学杂志》2017年第2期148-153,共6页Journal of Forensic Medicine

基　　金：十三五国家重点研发计划项目(2016YFC0800704);上海市科委资助项目(15dz1207500);上海市法医学重点实验室资助项目(17DZ2273200);上海市司法鉴定专业技术服务平台资助项目(16DZ2290900)

摘　　要：目的建立血液中硫离子的气相色谱-质谱(GC-MS)测定方法,并将其应用于实际案例。方法取血液0.2 mL,以1,3,5-三溴苯为内标,经五氟苄基溴衍生化后采用GC-MS分析。结果血液中的硫离子在0.2~40μg/mL质量浓度范围内线性良好,最低检出限为0.05μg/mL。健康人群、死后等不同来源的空白血液中硫离子质量浓度不高于0.05μg/mL。3例硫化氢中毒案件中,6名死者血液中均检出硫离子,质量浓度在1.02~3.13μg/mL。结论本研究建立了血液中硫离子测定方法,并成功应用于硫化氢中毒死亡案件。Objective To establish a gas chromatography-mass spectrometry （GC-MS） method for the determination of sulfide ion in blood and apply it to the practical cases. Methods The 1,3, 5 -tribro- mobenzene was selected as an internal standard, and 0.2 mL blood sample was collected and analyzed using GC-MS after a-Bromo-2,3,4, 5, 6-pentafluorobenzyl bromide derivatization. Results The mass concentration of sulfide ion in blood had good linearity in the range of 0.2-40μg/mL with a limit of detection （LOD） of 0.05μg/mL. The mass concentration of sulfide ion was less than 0.05 μg/mL in blank blood from different sources such as healthy subjects and dead cases. In 3 sulfide poisoning cases, sul-fide ion was detected in the blood samples of 6 victims, and the mass concentration range was 1.02-3.13μg/mL Conclusion This study establishes a method for investigation of sulfide ion in blood which has been applied successfully to the cases of fatal sulfide poisonings.

关 键 词：法医毒理学 中毒 硫化氢 衍生化 气相色谱-质谱联用法 

分 类 号：R595.4[医药卫生—内科学]