人参二醇、人参三醇组分的结构修饰及其产物对B16-F10肿瘤细胞的抑制作用  

Structural modification of panaxadiol and panaxatriol components and its inhibition on B16-F10 tumor cells

作　　者：顾永吉 刘墨祥[1] 

机构地区：[1]扬州大学医学院,江苏扬州225001

出　　处：《扬州大学学报（自然科学版）》2017年第1期38-41,共4页Journal of Yangzhou University：Natural Science Edition

基　　金：国家自然科学基金资助项目(81001589);江苏省普通高校研究生科研创新计划项目(CXLX11_1037)

摘　　要：以人参二醇、人参三醇组分为反应底物,采用组合化学的方法利用催化剂H对其结构进行修饰,获得具有抑制B16-F10细胞增殖的产物.分别采用快速制备液相色谱法与薄层色谱法分离、鉴定反应产物组分,并运用MTT比色法测定其体外抗肿瘤活性.结果表明,人参二醇、人参三醇组分与催化剂H反应72h后可得到极性比底物大的产物,经分离纯化可得到产物1、产物2、产物3等3个组分,其中产物3极性较大,产物1、产物3能明显增强体外抗肿瘤活性,产物2的体外抗肿瘤活性未明显增强.The panaxadiol and panaxatriol components are used as the substrate and are modified by the catalyst H with the method of combination chemistry.The products of the reaction are purified and identified by pre-HPLC method and thin layer chromatography,and its antitumor effects in vitro are determined by MTT assay.The results show that higher polar products are detected by thin layer chromatography after 72 hreaction of panaxadiol and panaxatrial components along with the catalyst H.The reaction products can be purified by liquid chromatography and three components(1,2,3)can be obtained.Among them,product 3has a higher polarity,products 1and product 3can significantly enhance the anti-tumor activities in vitro.Yet the anti-tumor activity of product 2is not significantly enhanced in vitro.

关 键 词：人参二醇 人参三醇 组合化学 抗肿瘤活性 

分 类 号：R284[医药卫生—中药学]