新藤黄酸通过内质网应激诱导人鼻咽癌CNE-2Z细胞凋亡的研究  

Gambogenic acid induces apoptosis of human nasopharyngeal carcinoma CNE-2Z cells through endoplasmic reticulum stress

作　　者：徐婷[1] 李庆林[1] 

机构地区：[1]安徽中医药大学新安医学教育部重点实验室,安徽合肥230038

出　　处：《安徽医药》2017年第3期439-443,共5页Anhui Medical and Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(81173600;81673650)

摘　　要：目的探讨新藤黄酸(gambogenic acid,GNA)对人鼻咽癌CNE-2Z细胞的影响和相关分子机制。方法采用倒置显微镜观察药物处理组和对照组的CNE-2Z细胞形态的变化;MTT法检测药物处理组的CNE-2Z细胞增殖的抑制作用;DAPI染色和AV/PI双染实验观察细胞凋亡情况;蛋白质印迹法检测GRP78、CHOP和ATF4蛋白的表达。结果倒置显微镜和荧光显微镜观察,发现与对照组相比,GNA作用CNE-2Z细胞后,体积缩小变圆,细胞核发生典型核染色质固缩等细胞凋亡形态学的变化。GNA可以抑制人鼻咽CNE-2Z细胞的增殖,并有时间和浓度依赖性。AV/PI染色后早凋细胞膜呈苹果绿色,晚凋或坏死细胞质呈不同程度的黄-红色。蛋白质印迹法检测表明GRP78蛋白表达总体呈现浓度依赖性下调,上调了CHOP蛋白和ATF4蛋白。结论 GNA可以影响人鼻咽癌CNE-2Z细胞的增殖,引发细胞凋亡,这可能与内质网应激相关蛋白(GRP78、CHOP和ATF4蛋白)有关。Objective To investigate the molecular mechanism by which gambogenic acid （GNA） induces the apoptosis of humannasopharyngeal carcinoma CNE-2Z cells. Methods Inverted microscope method was used to observe morphological changes of CNE-2Zcells in the drug treatment group and the control group. The inhibitory rate of human nasopharyngeal carcinoma CNE-2Z cellproliferation was determined by MTT assay in the drug treatment group. The cell apoptosis was determined by DAPI staining and AV/PIdouble staining. Western blot detected protein expression of GRP78, CHOP and ATF4 protein. Results Inverted microscopy andfluorescent microscopy showed cell volume shrinkage typical nuclear chromatin condensation and other morphological changes ofapoptosis compared with the control group. GNA can inhibit the proliferation of human nasopharyngeal carcinoma cell CNE-2Z,and hasthe time and concentration dependence. The AV/PI double staining indicated the early apoptotic cell membrane was apple green, thelate apoptosis or necrosis cytoplasm showed different levels of yellow-red. Western blot analysis showed that GNA can down-regulate theexpression of GRP78 protein and has concentration dependent, and it can up-regulate the expression of CHOP protein and ATF4protein. Conclusions GNA can inhibit proliferation and induce apoptosis in CNE-2Z c e lls,an d it may be related to the endoplasmicreticulum stress associated proteins, such as GRP78 ,CHOP, ATF4 protein.

关 键 词：鼻咽肿瘤 藤黄 内质网应激 

分 类 号：R285[医药卫生—中药学]