DPF2基因RNA干扰对人胰腺癌细胞PANC-1增殖、凋亡和细胞周期的作用研究  

Effect of DPF2-RNAi on proliferation,apoptosis and cell cycle of PANC-1 cells

作　　者：刘超[1] 孙如玉[1] 黄健[1] 刘丽华[2] 方圣云[1,3] LIU Chao SUN Ru-yu HUANG Jian LIU Li-hua FANG Sheng-yun(School of Basic Medical Sciences Institute of Clinical Pharmacology, Anhui Medical University,Hefei 230032,China Center for Biomedical Engineering and Technology（BioMET）,University of Maryland, MD Baltimore 21201,USA)

机构地区：[1]安徽医科大学基础医学院,安徽合肥230032 [2]安徽医科大学临床药理研究所,安徽合肥230032 [3]美国马里兰大学生物医学工程技术中心,马里兰巴尔的摩21201

出　　处：《中国药理学通报》2017年第5期647-653,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 31271159);安徽省高校学科拔尖人才学术资助重点项目(No gxbj ZD2016030)

摘　　要：目的已知DPF2参与白血病以及肿瘤的发生,但是DPF2是否参与胰腺癌发生和进展还不清楚,因此观察了DPF2基因RNA干扰对胰腺癌细胞系PANC-1细胞增殖、凋亡和细胞周期的影响。方法采用慢病毒介导的DPF2基因RNA干扰敲低PANC-1细胞的DPF2表达,通过克隆形成实验和MTT实验检测DPF2基因RNA干扰对PANC-1细胞增殖的作用,通过流式细胞术检测DPF2基因RNA干扰对PANC-1细胞凋亡和细胞周期的作用。结果慢病毒介导的DPF2基因RNA干扰中剂量和高剂量(2μL和4μL)使PANC-1细胞的DPF2表达明显降低。与阴性对照组比较,DPF2基因RNA干扰明显抑制PANC-1细胞活力和克隆形成,还促进PANC-1的凋亡。此外,DPF2基因RNA干扰引起细胞周期的S期阻滞,明显减少G2/M周期的细胞数量。结论 DPF2可能参与胰腺癌细胞PANC-1的增殖、凋亡过程和细胞周期的调控,通过慢病毒介导的DPF2基因RNA干扰敲低DPF2蛋白表达,可能为寻找潜在的抗胰腺癌的新方法提供实验依据。Aim DPF2 has been reported to be involved in pathogenesis of leukaemia and oncogenic activity.However, the role of DPF2 in oncogenesis and pathogenesis of pancreatic cancer remains unclear.Therefore, the present research aims to investigate the effects of DPF2-RNAi on proliferation, apoptosis and cell cycle regulation of a pancreatic cell line, PANC-1 cells.Methods The lentivirus-mediated DPF2-RNAi was employed to knockdown DPF2 expression in PANC-1 cells, and the role of DPF2-RNAi in proliferation, apoptosis and cell cycle regulation of the PANC-1 cells was examined through MTT assay, colony formation assay and flowcytometry assay.Results The lentivirus-mediated DPF2-RNAi middle and high doses（2 μL and 4 μL）significantly decreased the expression of DPF2 in the PANC-1 cells.DPF2-RNAi decreased cell viability and colony formation, and increased apoptosis of the PANC-1 cells.Besides, DPF2-RNAi induced the S-phase arrest and decreased G2/M phase population of the PANC-1 cells.Conclusions DPF2 may play a crucial role in proliferation, apoptosis and cell cycle regulation of PANC-1 cells.Knockdown of DPF2 through lentivirus-mediated DPF2-RNAi may provide experimental basis for finding a new method for therapy of pancreatic cancer.

关 键 词：DPF2 RNA干扰 慢病毒 PANC-1 增殖 凋亡 细胞周期 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]