清气凉营颗粒抗流感病毒药效学研究和分子机制分析  

Analysis of anti-influenza virus pharmacodynamics of QingQiLiangYing granule and its molecular mechanism

作　　者：王旭[1,2] 余苏云[1,2] 沈培亮[1,2] 汪思亮[1,2] 韦忠红[1,2] 刘兆国[1,2] 王爱云[1,2,3] 陈文星[1,2,3] 陆茵[1,2,3] WANG Xu YU Su-yun SHEN Pei-liang WANG Si-liang WEI Zhong-hong LIU Zhao-guo WANG Ai-yun CHEN Wen-xing LU Yin(School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medicine, Nanjing 210023, China Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine Prevention and Treatment of Tumor, Nanjing 210023, China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏省中药药效与安全性评价重点实验室,江苏南京210023 [3]江苏省中医药防治肿瘤协同创新中心,江苏南京210023

出　　处：《中国药理学通报》2017年第5期719-728,共10页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81673725;81573859;81673648;81403260);中国博士后科学基金(No 2014M551639);江苏省自然科学基金资助项目(No BK2012854);江苏省博士后资助项目(No 1401138C);2013年江苏高校优秀科技创新团队计划;江苏高校品牌专业建设工程资助项目(No PPZY2015A070);江苏高校中药学优势学科建设工程资助项目(PAPD)

摘　　要：目的探讨清气凉营颗粒抗流感病毒药效学研究和分子机制分析。方法体内实验验证清气凉营颗粒抗甲型流感药效,同时利用活性成分筛选、靶点预测技术,结合生物信息学手段,确定清气凉营颗粒治疗流感的特异性靶点,并进行信号通路富集分析,探讨其治疗流感的分子机制。结果体内实验显示清气凉营颗粒具有抗甲型流感的作用,而其抗流感的分子机制主要参与了Wnt信号通路,调控细菌侵袭性上皮细胞信号通路,同时也影响了致病性大肠杆菌感染信号通路、军团杆菌病信号通路和甲型流感信号等通路。结论清气凉营颗粒主要从抑制细菌感染、抗病毒感染和抑制病毒复制3个方面抑制流感。Aim To analyse the anti-influenza virus pharmacodynamics of QingQiLiangYing ganule andexplore the possible mechanism in the treatment of influenza.Methods The anti-influenza effect of QingQiLiangYing granule was confirmed by in vivo experiment.Unique targets regulated by QingQiLiangYing granule were predicted using the systematic pharmacology and bioinformatics technology.Further pathway enrichment analysis was performed to research the potential mechanism of QingQiLiangYing curing influenza.Results The in vivo experiment showed that QingQiLiangYing granule had the anti-influenza effect and its molecular mechanism of anti-influenza was mainly involved in Wnt signaling pathway, regulation of bacterial invasion of epithelial cells, thus affecting the pathogenic Escherichia coli infection, Legionella disease and influenza A disease processes.Conclusion QingQiLiangYing granule inhibits influenza mainly from three aspects：anti-bacterial infection, anti-viral infection and inhibition of virus replication.

关 键 词：清气凉营颗粒 中药复方 流感 系统药理学 网络药理学 分子机制 

分 类 号：R282.71[医药卫生—中药学] R283.6[医药卫生—中医学]