微生物转化对山楂提取物挥发性香气成分的影响  

Influence of Biotransformation on the Volatile Aroma Components of Hawthorn Extract

作　　者：陈义坤[1] 罗诚浩[1] 魏敏[1] 李冉[1] 岳海波[1] 郭国宁[1] CHEN Yi-kun LUO Cheng-hao WEI Min LI Ran YUE Hai-bo GUO Guo-ning(Technology Center of China Tobacco Hubei Industry Limited-liability Company, Wuhan 430040, Hubei, Chin)

机构地区：[1]湖北中烟工业有限责任公司技术中心,湖北武汉430040

出　　处：《食品研究与开发》2017年第7期149-153,共5页Food Research and Development

摘　　要：为了研究食甲醇芽孢杆菌(Bacillus methylotrophicus)J4-1生物转化对山楂提取物挥发性成分的影响,采用同时蒸馏萃取、GC/MS对比分析经回流提取后由J4-1转化的山楂提取物和对照的挥发性成分。结果表明:1微生物转化山楂提取物鉴定出58种挥发性成分,新增35种,包括己酸、乙酸己酯、丁酸己酯、松油醇、二氢猕猴桃内酯、光黄素等,占总相对百分含量21%以上;对照样品鉴定出34种挥发性成分;2相对百分含量显著增加的有己醛、糠醛、葵醛、棕榈酸乙酯、柠檬酸三丁酯等15种,刺激性成分如邻苯二甲酸异丁酯、邻苯二甲酸单(2-乙基己基)酯显著降低,14种挥发性成分消失。经微生物转化的山楂提取物挥发性成分改变大,具有酸甜、奶香丰富香气,刺激性成分减少,香气质提高。To Study the influence of biotransformation on the volatile components of hawthorn extract, the con-stituents in the biotransformed hawthorn extract and its contrast were analyzed by GC/MS after simultaneous distillation. Results： ①58 volatile components were identified from the biotransformed hawthorn extract, in-cluding 35 kinds of new： hexanoic acid,acetic acid ester,hexyl butyrate,terpinol,dihydroactinidiolide,lumi-flavine, etc., which accounted for 21%of the total volatile components of the biotransformed hawthorn extract;and 34 kinds of volatile components were identified from the control samples; ②15 kinds of volatile compo-nents＇ relative percentage content increased significantly, including hexanal,furfural,decanal,hexadecanoic acid, ethyl palmitate,tributyl citrate, etc.;Irritating ingredients such as 1,2-Benzenedicarboxylic acid, butyl 2-methylpropyl ester and 1,2-Benzenedicarboxylic acid, mono （2-ethylhexyl） ester decreased significantly;14 kinds of volatile components disappeared. After biotrasformation, the volatile components of hawthorn extract has changed greatly, with sweet and sour, milk fragrance rich aroma, and stimulating ingredients reduce、im-proving the quality of aroma.

关 键 词：食甲醇芽孢杆菌(J4-1) 微生物转化山楂提取物 GC/MS 挥发性香气成分 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]