口服活性AdipoRon对2型糖尿病小鼠肝脏氧化应激的干预作用  

Intervention effects of oral active AdipoRon on liver oxidative stress in type 2 diabetic mice

作　　者：肖敏[1] 屈小虎 陈慧[1] 蔡强军[1] 谢克俭[1] 

机构地区：[1]温州医科大学检验医学院与生命科学学院,浙江温州325035

出　　处：《中国应用生理学杂志》2017年第2期124-127,135,共5页Chinese Journal of Applied Physiology

基　　金：2015年度浙江省公益技术应用研究计划(实验动物)项目(2015C37099)

摘　　要：目的:研究口服活性AdipoRon对2型糖尿病小鼠肝脏氧化应激是否有干预作用,为临床应用提供基础资料。方法:将健康雄性C57BL/6小鼠分为正常组(n=8),糖尿病组(n=8),AdipoRon高剂量治疗组(n=8),AdipoRon低剂量治疗组(n=8),以高脂饲料喂养6周后腹腔注射40 mg/kg链脲佐菌素(STZ)诱导2型糖尿病模型,用高、低剂量的口服活性AdipoRon分别对治疗组灌胃治疗10 d后,检测相关生化指标,Western-blot法检测肝脏组织中IRS-1蛋白的表达;实时荧光定量PCR检测胰腺组织中PDX-1 mRNA的表达。结果:DM组小鼠血糖值明显高于NC组(P<0.05),DM+L组和DM+H组小鼠血糖值显著低于DM组。DM组小鼠肝脏组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性显著低于NC组(P<0.05),丙二醛(MDA)及一氧化氮合酶(NOS)活性显著高于NC组(P<0.05);DM+L组和DM+H组SOD、CAT活性明显高于DM组(P<0.05),MDA及NOS活性显著低于DM组(P<0.05)。肝脏组织中IRS-1的蛋白表达及胰腺PDX-1 mRNA表达显著升高,存在统计学意义(P<0.05)。结论:口服活性AdipoRon对糖尿病小鼠肝脏组织氧化应激有一定的干预作用,能降低小鼠的血糖水平。Objective： To explore the intervention effects of oral active AdipoRon on liver oxidative stress in type 2 diabetic mice, which provides basic data for clinical application. Methods： Thirty-two healthy male C57BL/6 mice were divided into 4 groups： normal group （NC, n = 8）, diabetes mellitus group （DM, n = 8）, high dose AdipoRon treatment group （DM ＋ H, n = 8） and low dose AdipoRon treatment group （DM ＋ L, n = 8）. Following six weeks high fat feed, mice of DM, DM ＋ H and DM ＋ L were intraperitoneally injected with 40 mg/kg streptozocin （STZ）, leading to type 2 diabetes. Afterwards, DM ＋ H group and DM ＋ L group were continuously treated with high dose and low doses of oral AdipoRon respectively for 10 days, following which, related biochemical indicators were detected. Western blot method was used to detect the p-IRS-1 protein expression in liver tissue and RT- PCR method to detect PDX-1 mRNA expression in the pancreas. Results： The blood glucose of DM group was obviously higher than that of NC group （ P 〈 0.05）. Compared to that of DM group, blood glucose of DM ＋ H group as well as DM ＋ L group was significantly lower. Activity of superoxide dismutase （SOD）, catalase （CAT） in liver tissue of DM mice was significantly lower than that of NC group （P 〈 0.05）; activity of malondialdehyde （MDA） and nitric oxide synthase （NOS） in DM group significantly higher than that of NC group （ P 〈 0.05） ; activity of SOD and CAT in DM ＋ L group and DM ＋ H group obviously higher than DM group （ P 〈 0.05） ; activity of MDA and NOS in DM ＋ L group and DM ＋ H group significantly lower than DM group （ P 〈 0.05）. And the p- IRS-1 protein expression in liver tissue and PDX-1 mRNA level in pancreas increased significantly （ P 〈 0.05）. Conclusion： Oral active AdipoRon which reduced the blood glucose levels of mice had a certain intervention effect on liver tissue oxidative stress in type 2 diabetes mice.

关 键 词：AdipoRon 2型糖尿病小鼠 肝脏 氧化应激 

分 类 号：R587.1[医药卫生—内分泌]