基于液固耦合的光镊拉伸红细胞变形特性  

Deformation of red blood cells stretching by optical tweezers based on fluid-structure interaction

作　　者：王带领 谭建平[1] 程立志[1] 喻哲钦 

机构地区：[1]中南大学机电工程学院,湖南长沙410083

出　　处：《中国医学物理学杂志》2017年第4期404-409,共6页Chinese Journal of Medical Physics

基　　金：国家自然科学基金(51475477;31271057)

摘　　要：目的:通过液固耦合的方法模拟红细胞在光镊拉伸过程中的变形情况,从而得到其剪切模量等力学参数,以探索其变形规律。方法:通过PRO/E建立血细胞三维双凹椭球模型,导入到ABAQUS软件中,采用超弹性材料,通过流体腔模型实现液固耦合,对光镊拉伸红细胞模型进行有限元计算。仿真再现红细胞变形的整个过程,拟合Suresh试验数据得到红细胞的剪切模量,进一步分析细胞质、细胞大小和形状与变形能力的关系。结果:仿真得到正常红细胞的剪切模量在5~9μN/m之间;细胞形状和直径均对变形能力有一定影响:直径越大,变形能力越差;双凹椭球细胞相对于球形细胞有更好的变形能力。结论:流体腔模型能够高效实现液固耦合,较好地模拟红细胞在光镊拉伸过程中的变形情况,直观地揭示细胞质、细胞大小和形状与变形的关系。为研究其他壳液提供了一种方法和参考。Objective To obtain the shear modulus and other mechanical parameters of red blood cells, and to explore the deformation rule by using the fluid-structure interaction method to simulate the deformation of red blood cells in the optical tweezers stretching process. Methods The three-dimensional double concave ellipsoid model of the blood cells was established by using PRO/E, and was transferred into ABAQUS software. With the use ofhyperelastic material, fluid-structure interaction was conducted based on fluid cavity model. Finite element calculation was performed for the model of red blood cells stretched by optical tweezers. The whole process of red blood cells deformation was reproduced in the simulation. The shear modulus of red blood cells was obtained by fitting the experimental data from Suresh. The relationship among cytoplasm, the size and shapc of the cell, and the deformation ability was analyzed. Results The simulation showed that the shear modulus of normal red blood cells was 5-9 μN/m. The cell shape and diameter had some influence on the deformation ability. The larger the diameter was, the worse the deformation ability became, and double concave cells showed better deformation ability than spherical cells. Conclusion The fluid cavity model is effective in achieving the fluid-structure interaction and simulating the deformation of red blood cells in the optical tweezers stretching process. The relationship among cytoplasm, the size and shape of cells, and deformation is revealed, which provides a new way and reference for the study of other shell liquid.

关 键 词：光镊 红细胞 ABAQUS 流体腔 液固耦合 

分 类 号：R318.01[医药卫生—生物医学工程]