骨松健骨方对骨质疏松模型大鼠股骨骨密度、力学结构的影响  

Efficacy of GSQGD(骨松健骨方) on Bone Density and Mechanical Structure in Rats with Osteoporosis

作　　者：毛春焕[1] 孔西建[1] 毛书歌[1] 王咪娜[1] 范鑫梅 张明柱 MAO Chun-huan KONG Xi-jian MAO Shu-ge WANG Mi-na FAN Xin-mei ZHANG Ming-zhu(Luoyang Bone-setting Hospital of He＇nan Province/He＇nan Orthopedics Hospital, Luoyang He＇nan 471002, China Zhongzhou People＇s Hospital of Laocheng District, Luoyang He＇nan 471002, China)

机构地区：[1]河南省洛阳正骨医院/河南省骨科医院,河南洛阳471002 [2]洛阳市老城区中州人民医院,河南洛阳471002

出　　处：《中医药导报》2017年第7期30-34,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：河南省中医药科学研究专项课题(2013zy04058);项目名称:中医中药治疗原发性骨质疏松症的研究

摘　　要：目的:研究骨松健骨方对骨质疏松模型大鼠股骨骨密度、力学结构的影响,探讨骨松健骨方治疗骨质疏松的作用机制。方法:采用去势雌性大鼠作为骨质疏松模型,将其分为假手术组、模型组、雌二醇组、骨松健骨方低、中、高剂量组,假手术组、模型组灌胃生理盐水,雌二醇组灌胃雌二醇,骨松健骨方低、中、高剂量组灌胃不同剂量骨松健骨方,给药12周后,检测大鼠股骨的骨密度和骨矿含量,三点弯曲实验检测大鼠股骨的生物力学性能,Micro-CT检测股骨远端的骨小梁数目及形态等变化情况。结果:给药后,E_2组、骨松健骨方低、中、高剂量组大鼠体质量不同程度低于模型组(P<0.05或P<0.01),且呈剂量依赖性;E_2组和骨松健骨方中剂量、高剂量组均能显著抑制大鼠股骨BMD的下降(P<0.01),呈剂量依赖性;骨矿物含量(BMC)在各组之间未见明显变化;E_2组及骨松益强方中、高剂量组可不同程度地提高大鼠骨应力、弹性模量(P<0.05或P<0.01)及最大载荷(P<0.05)。结论:骨松健骨方可提高去势大鼠的骨强度,防止大鼠雌激素减少所引起的体质量增加,增加大鼠骨密度,改善骨显微结构,增加骨小梁数目,降低骨小梁间隙,增强骨骼生物力学性能,对去势大鼠的骨丢失具有较好的防治作用。Objective： To study the efficacy of GSQGD （骨松健骨方） on bone density and mechanical structure in rats with osteoporosis. Methods： Ovariectomized rats were used as a model of osteoporosis, and divided into sham operation group, model group, estrogen group, low, middle and high doses GSQGD groups. The sham operation group and ovariectomized group were given saline gavage, and estradiol group with estradiol. The low, middle and high doses GSQGD groups were administrated with different doses of GSQGD. The detection of rat femur bone mineral density and bone mineral content, the biomechanical properties of three point bending test of rat femur, trabecular number and morphology changes o~ Micro-CT detection of distal femur were observed, in 12 weeks, Results： The estrogen group, low, middle and high doses GSQGD groups showed lower weight than model group （P〈0.05 or P〈0.01）, in a dose-dependent manner; E2 group, middle and high doses GSQGD groups inhibited the decline of femur BMD （P〈0.01）, in a dose-dependent manner; bone mineral content （BMC） among the groups showed no obvious change; E2 group, middle and high doses GSQGD groups could increase the rat bone stress and elastic modulus （P〈0.05 or P〈0.01） and the maximum load （P〈 0.05）. Conclusion： GSQGD can improve bone strength in ovariectomized rats, reduce the increase of body weight caused by estrogen, increase bone density, improve bone microstructure, improve the number of trabecular bones, reduce the bone trabecular space, and enhance the biomechanical properties of bone in ovariectomized rats, with prevention on bone loss.

关 键 词：骨松健骨方 骨密度 去势大鼠 骨显微结构 骨生物力学性能 

分 类 号：R285.5[医药卫生—中药学]