HPLC-ELSD法测定特制接骨丸中三七皂苷R_1、人参皂苷Rg_1、Re的含量  

Determination of Notoginsenoside R1, Ginsenosides Rg1 and Re in Tezhi Jiegu Pills(特制接骨丸) By HPLC-ELSD

作　　者：杜志谦[1,2] 张利静[2] 夏华玲 马守芳 郑超[2] DU Zhi-qian ZHANG Li-jing XIA Hua-ling MA Shou-fang ZHENG Chao(Institute of Orthopedics and TraumatoIogy of He＇nan Province, Luoyang He＇nan 471000, China He＇nan University of Traditional Chinese Medicine, Zhengzhou He＇nan 450008, China)

机构地区：[1]河南省正骨研究院,河南洛阳471000 [2]河南中医药大学,河南郑州450008

出　　处：《中医药导报》2017年第7期77-79,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:建立HPLC-ELSD法测定特制接骨丸中三七皂苷R_1、人参皂苷Rg_1和人参皂苷Re3种皂苷含量的分析方法。方法:采用HPLC-ELSD法,色谱柱为Wonda Sil C_(18)柱(4.6 mm×250 mm,5μm);流动相:乙腈(A)-水(B),梯度洗脱(0~10 min,19%→21%A;10~19 min,21%→23%A;19 min^20 min,23%→32%A;20~28 min,32%A;28~29 min,32%→33%A;29~40 min,33%A;40~41 min,33%→19%A;41~48 min,19%A);柱温:25℃;流速:1.0m L·min^(-1);ELSD检测器参数:漂移管温度为108℃,气体流量为2.8 L·min^(-1)。结果:三七皂苷R_1、人参皂苷Rg_1、人参皂苷Re分别在进样量为0.515~1.030、2.500~5.000、1.510~3.020μg线性关系良好,三七皂苷R_1、人参皂苷Rg_1、人参皂苷Re平均加样回收率分别为99.67%、97.49%、101.74%。结论:HPLC-ELSD法可以准确、快速地实现特制接骨丸中多种皂苷类成分的定量测定,从而用于特制接骨丸的质量控制。Objective： To establish a determination of the content of Tezhi Jiegu Pills notoginsenoside R1, ginsenosides Rgland ginsenosides Re by HPLC-ELSD. Methods： HPLC-ELSD was used. The analysis was car- ried on WondaSil C18 ODS （4.6 mm×250 mm, 5 μm） column; the mobile phase was the acetonitrile （A）-water （B）; the gradient elution （0-10min, 19%→21% A; 10-19rain, 21%→23% A; 19min-20min,23%→32% A; 20-28 rain, 32% A; 28-29 rain, 32%→33% A; 29-40 min, 33% A; 40-41 min, 33%→19% A; 41-48 min, 19% A）; the col- umn temperature was 25 ℃;The flow rate was 1.0 mL·min^-1; The drift tube temperature was 108 ℃; the gas flow was 2.8 L＇min＂. Results： Notoginsenoside R1, ginsenosides Rgland ginsenosides Re respectively in 0.515-1.030, 2.500-5.000 and 1.510-3.020 μg good linearity in the range. The average recovery and RSD of notoginsenoside R1, ginsenosides Rgland ginsenosides Re respectively were 99.67%, 97.49% and 101.74%. Conclusion： HPLC- ELSD method can accurately and quickly realize the quantitative determination of the saponins in the s Tezhi Jiegu Pills, and it can be used for control the quality.

关 键 词：特制接骨丸 三七皂苷R1 人参皂苷Rg1、人参皂苷Re 含量测定 

分 类 号：R284[医药卫生—中药学]