氧化应激条件下克癃胶囊对PC-3细胞内GSH、ROS水平的影响  

Effects of Kelong capsules on glutathione and reactive oxygen species levels in PC-3 cells under oxidative stress

作　　者：全丹妮 张玲[1] 颜苗[2,3] 樊新荣[1,4] 

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]中南大学湘雅二医院 [3]中南大学临床药学研究所,湖南长沙410011 [4]中国中医科学院,北京100700

出　　处：《湖南中医杂志》2017年第4期138-140,共3页Hunan Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81373708;81202985;81573686)

摘　　要：目的:研究克癃胶囊对双氧水诱导的PC-3细胞氧化应激反应的保护作用。方法:体外培养PC-3细胞,建立双氧水诱导PC-3细胞氧化应激的模型,给予克癃胶囊进行干预。分为4组:正常组、模型组(H_2O_2 100μmol/L)、阴性对照组(tBHQ50μmol/L+H_2O_2100μmol/L)、克癃胶囊组(克癃方100μg/m L+H_2O_2 100μmol/L)以DCFH-DA为荧光探针,利用流式细胞仪检测细胞内活性氧自由基(ROS)含量,利用化学显色法测定PC-3细胞内还原型谷胱甘肽(GSH)含量,评价细胞氧化应激水平。结果:ROS、GSH水平模型组与正常组比较,阳性对照组、克癃胶囊组与模型组比较,差异均有统计学意义(P<0.01);ROS水平阳性对照组与克癃胶囊组比较,差异有统计学意义(P<0.01),但两组GSH水平比较,差异无统计学意义(P>0.05)。结论:克癃胶囊对双氧水诱导的PC-3细胞氧化应激具有保护作用。Objective ： To investigate the protective effect of Kelong capsules against the oxidative stress response of PC - 3 cells induced by hydrogen peroxide. Methods ： PC - 3 cells were cultured in vitro and treated with hydrogen peroxide to establish an oxidative stress model. Kelong capsules were given for intervention. PC -3 cells were divided into normal group, model group （H2O2 100μmol/L）, positive control group （tBHQ 50μmol/L ＋ H2O2 100μmol/L） ,and Kelong capsule group （Kelong capsules 100μg/mL ＋ H202 100μmol/L） With DCFH - DA as the fluorescent probe, flow eytometry was used to measure the reactive oxygen species （ROS） production in PC -3 cells. Chemical development was used to measure the level of glutathione （GSH） in PC - 3 cells and to evaluate intracellular oxidative stress. Results ：The ROS and GSH levels showed significant differences between the model group and normal group and between the positive control group, Kelong capsule group, and model group （P 〈0. 01 ）. There was a significant difference in ROS level between the positive control group and Kelong capsule group （P 〈 0. 01 ）, but no significant difference was found in GSH between the two groups （P 〉 0. 05 ）. Conclusion ： Kelong capsules can protect PC -3 cells against oxidative stress induced by hydrogen peroxide.

关 键 词：克癃胶囊 活性氧自由基 还原型谷胱甘肽 氧化应激 实验研究 

分 类 号：R285.5[医药卫生—中药学]