茯苓菌液态发酵合成茯苓三萜的实验研究  

Liquid-state fermentation of Poria cocos strains for the synthesis of Poria cocos triterpene: an experimental study

作　　者：廖彦[1] 易刚强[1] 陈林[1] 刘莎[1] 崔培梧[1] 奉家豪 周武林[1] 黄绍国[1] 鲁耀邦[1] 

机构地区：[1]湖南中医药大学,湖南长沙410208

出　　处：《湖南中医杂志》2017年第4期162-165,共4页Hunan Journal of Traditional Chinese Medicine

基　　金：湖南省发改委科研计划项目(湘发改高技[2014]1199号);湖南省中医药科研计划重点项目(编号:201621);湖南省教育厅科研计划项目(编号:16C1219);湖南省中药粉体与创新药物省部共建国家重点实验室培育基地开放基金项目(编号:ZYFT201404);湖南中医药大学研究性学习和创新性实验计划项目(编号:201616);湖湘中药资源保护与利用协同创新中心开放基金项目(编号:00175-12)

摘　　要：目的:探索茯苓菌液态发酵合成茯苓三萜的工艺条件。方法:从碳源、氮源、金属离子、辅助因子及初始pH角度对茯苓菌液态发酵合成茯苓三萜的过程进行系统分析,以确定高效发酵工艺。结果:最佳碳源为葡萄糖、氮源为玉米浆粉、添加金属离子ZnSO_4、MnSO_4、CuSO_4或FeSO_4以及辅助因子2-硫代巴比妥、初始pH范围为5~6。结论:通过培养基和发酵条件优化设计可显著提高茯苓三萜的合成效率。Objective ：To investigate the process eonditions for the liquid -state fermentation of Poria cocos strains for the synthesis of Poria cocos triterqene. Methods：A systematic analysis was performed for the process of liquid - state fermentation of Poria cocos strains to synthesize Poria cocos triterpene from the perspectives of carbon source, ni- trogen source,metal ions, eofaetors, and initial pH value. Results：The best carbon source was glucose and the best nitrogen source was corn steep powder. The metal ions added were ZnSO4, MnSO4, CuSO4 ,or FeSO4, and the eofaetor was 2 - thiobarbituric acid. The range of initial pH value was 5 - 6. Conclusion ： The optimized design of medium and fermentation conditions can significantly improve the efficiency of the synthesis of Poria cocos triterpene.

关 键 词：茯苓 三萜 生物合成 发酵调控 实验研究 

分 类 号：R284.3[医药卫生—中药学]