LOX-1介导oxLDL诱导的人脐静脉内皮细胞高通透性反应  

LOX-1 is Involved in the Increased Permeability Induced by oxLDL in Umbilical Vein Endothelial Cells

作　　者：李媛彬[1] 丘继哲[1] 何志军[1] 黄旭[1] 彭微[1] 陈壮[1] 

机构地区：[1]湖南中医药高等专科学校医学系,株洲412012

出　　处：《医学研究杂志》2017年第4期61-64,77,共5页Journal of Medical Research

基　　金：湖南省教育厅基金资助项目(15C1073)

摘　　要：目的探讨凝集素样氧化低密度脂蛋白受体1(lectin like oxidized low density lipoprotein receptor 1,LOX-1)在氧化低密度脂蛋白(oxLDL)诱导的血管内皮高通透性反应中的作用。方法采用Transwell单层细胞模型系统和油红O染色,观察oxLDL或和LOX-1阻断抗体对单层血管内皮细胞LDL通透性的影响以及下室THP-1巨噬细胞内脂质的蓄积情况;用Western blot法和激光扫描共聚焦显微镜检测oxLDL或和LOX-1阻断抗体对桥粒芯糖蛋白1(DSG1)在人脐静脉内皮细胞(HUVECs)中表达和分布的改变。结果 oxLDL可以增加单层内皮细胞对LDL的通透性和下室THP-1巨噬细胞脂质的蓄积,同时降低DSG1的表达及其在细胞间分布的连续性,而抑制LOX-1可以减弱内皮细胞的高通透性反应和巨噬细胞脂质蓄积。结论 oxLDL可能是通过降低桥粒蛋白表达使内皮细胞构型改变来诱导内皮高通透性,从而促进内皮下巨噬细胞内的脂质蓄积,其机制可能涉及LOX-1的介导。Objective To study the effect of （ lectin like oxidized low density lipoprotein receptor 1, LOX - 1 ） on the permeability injury associated with desmosomes protein disruption induced by oxidatively modified low- density lipoprotein （oxLDL） in human umbilical vein endothelial cells （HUVECs）. Methods We investigated the effect of oxLDL and/or LOX - 1 blocking antibody（10μg/ml） on endothelial monolayer integrity by using the methods of LDL across the endothelial monolayer, and lipid accumulation of THP - 1 macrophage with rathonum red O dyeing, and inmmunoreactivities of desmogleinl （DSG1） by using western blot and laser scanning confocal mi- croscope （LSCM）. Results The results showed that oxLDL can increase the LDL permeability of monolayer endothelial cells and lipid accumulation of THP - 1 macrophage, reduce the expression of desmosomes protein DSG1 in HUVECs, which was inhibited by the LOX - lblocking antibody（ 101xg/ml）. Conclusion These findings suggest that changes in expression of desmosome cadherins, DSG1, are involved in ox - LDL - induced compromise of the barrier properties of the vascular endothelium. In this process, activation of the LOX - lplays an important role.

关 键 词：氧化低密度脂蛋白 凝集素样氧化低密度脂蛋白受体1 桥粒芯糖蛋白1 内皮细胞通透性 脂质蓄积 

分 类 号：R3[医药卫生—基础医学]