斑马鱼血管内脂代谢研究方法的构建  

Establishing methods for studying intravascular lipid metabolism of zebrafish

作　　者：陈侃[1] 王长谦[1] 范虞琪[1] 韩志华[1] 汪月[1] 高霖[1] 曾华盨 CHEN Kan WANG Chang-Qian FAN Yu-Qi HAN Zhi-Hua WANG Yue GAO Lin ZENG Hua-Su(Department of Cardiology, Shanghai Ninth People＇ s Hospital Affiliated to School of Medicine of Shanghai Jiao Tong University, Shanghai 200011, China)

机构地区：[1]上海交通大学医学院附属第九人民医院心内科,上海市200011

出　　处：《中国动脉硬化杂志》2017年第4期398-403,共6页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金资助(81500331);上海市进一步加快中医药事业发展三年行动计划(2014年-2016年)资助(ZY3-CCCX-3-3002)

摘　　要：目的构建系统的斑马鱼血管内脂代谢的研究方法。方法利用油红O染色、荧光探针标记、直接血脂检测等方法研究斑马鱼胚胎、幼鱼、成鱼等不同发育时期血管内的脂质代谢,以及不同饮食对斑马鱼血脂的影响。结果油红可将斑马鱼胚胎血管内的脂质染色,荧光探针可荧光标记斑马鱼胚胎血管内的胆固醇。喂食蛋黄液可升高斑马鱼幼鱼的血脂水平,油红O染色可反映这种血脂的变化。高胆固醇饮食可升高斑马鱼幼鱼血管内胆固醇水平,荧光探针可标记幼鱼血管内胆固醇的变化。血脂检测显示高脂饮食的斑马鱼血清总胆固醇和甘油三酯均高于普通饮食的斑马鱼。结论本研究利用油红O染色、荧光探针标记及血脂检测等技术构建了斑马鱼不同发育阶段血管内脂代谢的研究方法。Aim To establish systematic methods for studying intravascular lipid metabolism of zebrafish. Methods Oil red O staining, CholEsteryl BODIPY 542/563 C11 fluorescently labelling and blood lipids detecting were used to study intravascular lipid metabolism of embryo, larvae and adult zebrafish and the effect of different diet on blood lipid of zebrafish. Results Intravascular lipid of embryo zebrafish was stained by oil red O staining, and intravascular cholesterol was labeled by CholEsteryl BODIPY 542/563 C11. Increased intravascular lipid of larvae zebrafish incubated in egg yolk was stained by oil red O staining, and increased intravascular cholesterol of larvae zebrafish fed by high choles- terol diet was labeled by CholEsteryl BODIPY 542/563 C 11. The serum total cholesterol and triglyceride of adult zebrafish were obviously increased after 7 weeks fat feeding. Conclusion Oil red O staining, CholEsteryl BODIPY 542/563 C 11 fluorescently labelling and blood lipids detecting can be applied for zebrafish intravascular lipid metabolism research.

关 键 词：斑马鱼 脂代谢 油红O染色 荧光探针标记 

分 类 号：R541.4[医药卫生—心血管疾病]