微小RNA-3620在抗结核药物致肝损伤患者血浆中的表达及临床意义  

Expression of microRNA-3620 in blood plasma and its clinical significance in patients with anti-tuberculosis drug-induced bepatotoxicity

作　　者：谢平[1] 朱彤[1] 陈彩萍[1] 白茹[1] 赵晖[1] 朱伟星[1] 刘亮明[1] 

机构地区：[1]上海交通大学附属第一人民医院松江分院感染科,201600

出　　处：《中华传染病杂志》2017年第3期161-164,共4页Chinese Journal of Infectious Diseases

基　　金：国家自然科学基金（81070357,30660066）;上海市松江区科学技术攻关项目（15SJGG42）

摘　　要：目的了解微小RNA（miRNA）-3620在抗结核药物诱导肝毒性（anti—tuberculosis druginduced hepatotoxicity，ATDH）患者血浆中的表达水平。方法ATDH和非ATDH患者各35例，实时荧光定量PCR检测两组患者血浆中miRNA-3620的相对表达量，两组间比较采用t检验；根据miRNA-3620水平绘制受试者工作特征曲线，评估血浆中miRNA-3620在ATDH诊断中的价值。结果ATDH患者和非ATDH患者血浆中miRNA-3620表达量分别为1．65±1．43和0．71±0．45，差异有统计学意义（t=3．703，P〈0．01）。miRNA-3620表达水平的最佳截点为1．15，曲线下面积为0．71（95％CI：0．43～1．45），miRNA-3620诊断ATDH的敏感度为60．0％、特异度为82.9％、阳性预测值为77．8％、阴性预测值为67．4％，正确诊断ATDH21例，非ATDH29例，准确度为71．4％。结论ATDH患者血浆中miRNA-3620表达水平明显升高。Objective To investigate the expression of microRNA （miRNA） -3620 in the plasma of patients with anti-tuberculosis drug-induced hepatotoxicity （ATDH）. Methods A total of 35 patients with ATDH and 35 non-ATDH paired individuals were included in this study. Plasma miRNA-3620 levels were detected using reabtime Polymerase chain reaction. Comparison between two groups was done with t test. Receiver operation characteristic （ROC） curve analysis was performed to determine the diagnostic value of miRNA-3620 in ATDH. Results The relative expression of plasma miRNA-3620 of patients with ATDH and non-ATDH were 1. 65 ± 1. 43 and 0. 71 ± 0. 45, respectively, with significantly statistical difference （t=3. 703, P〈0.01）. The cut off value of miRNA-3620 expression was 1. 15 and the area under ROC curve were 0. 71 （95% CI： 0. 43-1. 45）. Based on this cutoff value, the sensitivity and specificity of miRNA-3620 in diagnosing ATDH were 60. 0% and 82. 9~/6, respectively; the positive predictive value was 77.8% and the negative predictive value was 67.4%. Twenty-one ATDH cases and 29 non-ATDH cases was correctly diagnosed, with the accuracy of 71.4%. Conclusion The expression of miRNA-3620 in plasma is significantly increased in ATDH patients.

关 键 词：微小RNA-3620 抗结核药物诱导肝毒性 结核 肺 

分 类 号：R52[医药卫生—内科学] R575[医药卫生—临床医学]