阿萨希毛孢子菌临床株与小鼠骨髓来源树突状细胞共培养体系的构建  

The Establishment of a Co-culture System of Clinical Isolates of Trichosporon asahii and Murine Bone Marrow-derived Dendritic Cells

作　　者：巴根[1] 吕雪莲[2] 王瑞丽[1] 敖俊红[1] 杨苏腾[1] 廖勇[1] 杨蓉娅[1] 

机构地区：[1]陆军总医院(第二军医大学临床医学院)皮肤科,北京100700 [2]首都医科大学附属安贞医院皮肤科,北京100029

出　　处：《中国皮肤性病学杂志》2017年第5期481-485,共5页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金面上项目(81571972;81471928)

摘　　要：目的建立并优化阿萨希毛孢子菌临床株(Trichosporon asahii,T.asahii)与野生型小鼠骨髓来源树突状细胞(Bone marrow-derived dendritic cell,BMDC)共培养体系。方法体外培养C57BL/6小鼠BMDC。用不同浓度比例的热灭活T.asahii与BMDC共培养,筛选最优浓度比例,用脂多糖(LPS)和β-葡聚糖(β-glucan)分别刺激BMDC,选择适宜阳性对照;利用优化的体系进一步检测BMDC表面共刺激分子的激活和相关细胞因子的产生情况。结果 BMDC与T.asahii比例为1∶5时最适宜作为共培养的比例,而β-glucan更适于作为阳性对照;小鼠BMDC能够对T.asahii进行抗原提呈,其通过增加IL-6和TNF-α的产生发挥抗真菌作用。结论该共培养体系能够有效检测小鼠BMDC对T.asahii的免疫效应。Objective To establish and optimize a co-culture system of clinical isolates of Trichosporon asahii（T.asahii）with bone marrow-derived dendritic cells（BMDC）from C57BL/6 mice.Methods Optimal ratio of BMDC to heat-killed T.asahii was obtained by co-culturing them in different ratios.Positive control was determined by assessing cytokine production following stimulation of BMDC with LPS and β-glucan.Expression levels of costimulatory molecules in BMDC and the production of cytokines by BMDC were assessed in the optimized co-culture system.Results The optimal ratio of BMDC to T.asahii was 1：5, and β-glucan was suitable for positive control.The murine BMDC could present the antigens from T.asahii, and exerted anti-T.asahii property via stimulating the productions of IL-6 and TNF-α.Conclusion The co-culture system is a reliable approach to investigate the immune response of murine BMDC against T.asahii.

关 键 词：阿萨希毛孢子菌 小鼠 树突状细胞 细胞因子 

分 类 号：R756.9[医药卫生—皮肤病学与性病学]