间歇性应用免疫调节剂对亚临床型生殖器疱疹患者外周血淋巴细胞的影响  

Effects of Intermittent Application of Immunomodulators on Peripheral Blood Lymphocytes in Patients with Subclinical Genital Herpes

作　　者：张婧[1] 邢倩倩[1] 杨凤元[1] 蒙坚[1] 蒋冬香[2] 程培华[1] 

机构地区：[1]桂林医学院附属医院皮肤性病科,广西桂林541000 [2]桂林医学院附属医院实验室,广西桂林541000

出　　处：《中国皮肤性病学杂志》2017年第5期526-528,530,共4页The Chinese Journal of Dermatovenereology

基　　金：广西桂林市科技局攻关项目(2013012010);广西自然科学基金(2013GXNSFAA019206)

摘　　要：目的探讨影响亚临床型生殖器疱疹患者机体免疫水平的相关因素,以及予免疫调节剂治疗后机体免疫状态的改善情况。方法用荧光定量PCR检测患者带毒情况,用流式细胞仪检测患者的免疫功能。比较带毒阳性组与带毒阴性组,频发组(复发≥6次/年)与少发组(复发<6次/年)以及使用免疫调节剂治疗前后患者的外周血淋巴细胞百分比。结果带毒阳性组CD3^+,CD4^+,CD4^+/CD8^+T细胞均低于带毒阴性组,差异有统计学意义(P<0.05),频发组CD3^+,CD4^+,CD4^+/CD8^+,NK细胞均低于少发组,CD8^+T细胞高于少发组,差异有统计学意义(P<0.05),治疗组CD3^+,CD4^+,CD4^+/CD8^+,NK细胞均低于对照组,CD8^+T细胞高于对照组,差异有统计学意义(P<0.05),治疗后CD3^+,CD4^+,CD4^+/CD8^+,NK细胞均高于治疗前,CD8^+T细胞低于治疗前,差异有统计学意义(P<0.05),在各种分组情况下比较B细胞数值变化,差异无统计学意义(P>0.05)。结论亚临床型GH患者存在免疫功能低下的情况,带毒及频发患者的免疫抑制更为严重,而给予联合应用免疫调节剂后免疫低下较前改善。Objective This study was to investigate the factors affecting immune status in patients with subclinical genital herpes, and the improvements in immune status after treatment with immunomodulators.Methods HSV viral carriers were identified using fluorescent quantitative polymerase chain reaction（qPCR）.Flow cytometry was used to evaluate the patients＇ immune function.Percentages of peripheral blood lymphocytes were compared in HSV＋ vs.HSV-, more（annual recurrence ≥6）vs.less（annual recurrence〈6）frequent recurrence, as well as before and after immunomodulator therapy.Results Lower percentages of CD3＋,CD4＋,CD4＋/CD8＋ T cells were found in HSV＋ group（P〈0.05 vs.HSV-）.Patients with more frequent recurrence showed significantly lower percentages of CD3＋,CD4＋,CD4＋/CD8＋,natural killer cells, and higher percentage of CD8＋T cells（P〈0.05 vs.annual recurrence〈6）.Patients in the treatment group displayed significantly lower percentages of CD3＋,CD4＋,CD4＋/CD8＋,natural killer cells, and higher percentage of CD8＋T cells in comparison with normal controls（P〈0.05）.Following the treatment, the proportions of CD3＋,CD4＋,CD4＋/CD8＋,natural killer cells increased while the proportion of CD8＋T cells decreased（P〈0.05 vs.pre-treatment）.There were no significant differences in B cells in all groups（P〉0.05）.Conclusion Patients with subclinical genital herpes exhibit compromised immunity.Patients with HSV＋ or more frequent recurrence display severe immunosuppression. Treatment with combination of immunomodulators can improve immune function in HSV＋ patients.

关 键 词：亚临床型生殖器疱疹 病毒脱排 流式细胞仪 匹多莫德 免疫调节 

分 类 号：R752.1[医药卫生—皮肤病学与性病学]