核仁素对糖尿病性心肌病小鼠心肌细胞凋亡的影响  

Effect of nucleolin on cardiac cell apoptosis in Type 2 diabetic cardiomyopathy mice

作　　者：刘梅冬[1] 孙俪[1] 蒋碧梅[1] 谭斯品[1] 刘可[1] 肖献忠[1] 

机构地区：[1]中南大学湘雅医学院病理生理学系,长沙410078

出　　处：《中南大学学报（医学版）》2017年第3期241-245,共5页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(81170113)~~

摘　　要：目的:探讨核仁素对糖尿病性小鼠心肌病心肌细胞凋亡的影响。方法:小鼠给予高脂高糖饲料喂养,第5,6周分别予链脲佐菌素60 mg/kg腹腔注射,构建2型糖尿病模型;实验分为4组:野生型小鼠对照组、核仁素转基因小鼠对照组、野生型小鼠糖尿病组、核仁素转基因小鼠糖尿病组;第8周末检测糖尿病相关指标;第20周末,采用HE染色观察心肌形态学改变;TUNEL染色和caspase-3活性检测心肌细胞凋亡程度。结果:糖尿病组小鼠空腹血糖值较野生型对照组明显升高;与野生型对照组相比,野生型糖尿病组小鼠心肌HE染色显示细胞排列紊乱,部分肌纤维出现断裂及溶解,TUNEL染色和caspase-3活性检测显示心肌细胞凋亡明显增加。与野生型糖尿病组小鼠相比,转基因糖尿病组小鼠心肌形态学改变明显减轻,心肌细胞凋亡明显减少。结论:核仁素过表达可抑制糖尿病性心肌病中心肌细胞凋亡的发生。Objective：To investigate the effect of nucleolin on cardiac cell apoptosis in Type 2 diabetic cardiomyopathy mice.Methods：Mice were fed with high-fat and high-sugar food for 20 weeks（mice were injected intraperitoneally with 60 mg/kg streptozotocin in the 5th and 6th weeks）to establish a mouse model of Type 2 diabetes.The mice were divided into 4 groups：a wild type（WT）control group,a nucleolin transgenic（TG）control group,a WT diabetic group,a TG diabetic group.Diabetesrelated indicators were detected at the end of the 8th week.At the end of the 20th week,HE staining was used to observe myocardial morphological changes;TUNEL staining and caspase-3 activity were used to detect the extent of apoptosis of cardiac myocytes.Results：The level of fasting blood glucose was significantly increased in the diabetic group than that in the control group.In WT diabetic group,myocardial disarrangement,fragmentation and dissolution were observed（determined by HE staining）;cellular apoptosis（determined by TUNEL staining and caspase-3 activity）also increased markedly in the WT diabetic group.Compared with the wild mice in the diabetic group,myocardial morphological changes and cardiac myocytes apoptosis were alleviated significantly.Conclusion：Nucleolin overexpression affectes the occurrence and development of diabetic cardiomyopathy through inhibition of cardiac myocyte apoptosis.

关 键 词：2型糖尿病心肌病 核仁素 细胞凋亡 转基因小鼠 

分 类 号：R587.2[医药卫生—内分泌]