电针对异氟醚诱导的阿尔茨海默病小鼠行为学改变的作用及机制  

Effect of Electro-acupuncture on Behavior Alterations in Mice with Isoflurane-induced Alzheimer's Disease and Its Mechanism

作　　者：赖凤娇 李向宇[2] 徐乐[2] 卢昌碒 季刚[2] 朱晓英[2] LAI Feng-Jiao LI Xiang-Yu XU Le LU Chang-Yin JI Gang ZHU Xiao-Ying(The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou 510080 Guangdong, China Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120 Guangdong, China)

机构地区：[1]广州中医药大学第二临床医学院,广东广州510080 [2]广东省中医院,广东广州510120

出　　处：《广州中医药大学学报》2017年第3期376-380,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省科技厅资助项目(编号:2011B031800070)

摘　　要：【目的】探讨电针对异氟醚引起的阿尔茨海默病(AD)APPswe/PS1d E9双转基因小鼠(AD小鼠)行为学改变、皮质β淀粉样前体蛋白(APP)和β淀粉样蛋白(Aβ)的表达以及皮质神经细胞凋亡的影响。【方法】选用6月龄AD小鼠及同龄野生型小鼠,随机分为野生对照组、AD组、异氟醚组和电针组(N=8)。电针组在异氟醚处理前进行连续3 d的电针处理,取百会、涌泉穴,15 min/d。异氟醚处理:电针组和异氟醚组吸入体积分数1.2%异氟醚4 h。采用Morris水迷宫测试小鼠的学习记忆能力,Western blot法检测皮质APP-C83、APP-C99及Aβ的表达,TUNEL法检测皮质细胞凋亡情况。【结果】(1)逃避潜伏期:AD组显著长于野生对照组,异氟醚组长于AD组,电针组短于异氟醚组(均P<0.05)。(2)目标象限停留时间百分比及穿越平台次数:异氟醚组均低于AD组(P<0.05),电针组高于异氟醚组(P<0.05)。(3)皮质APP-C83的表达:异氟醚组显著低于AD组(P<0.05),电针组高于异氟醚组(P<0.05)。(4)皮质APP-C99的表达:异氟醚组显著高于AD组(P<0.05),电针组低于异氟醚组(P<0.05)。(5)皮质凋亡指数:异氟醚组显著高于AD组(P<0.05),电针组低于异氟醚组(P<0.05)。(6)AD组、异氟醚组、电针组小鼠皮质Aβ的表达水平显著高于野生对照组(P<0.05)。【结论】电针可减轻异氟醚诱导的AD样病神经毒性,减轻异氟醚诱使的AD小鼠学习记忆能力的下降,可能与其抑制APP-C99的过量表达,减少Aβ的生成和积聚,进而减少神经细胞的凋亡有关。Objective To investigate the effect of electro-acupuncture on behavior alterations, the expression of amyloid precursor protein（APP）, amyloid β-protein（Aβ ） proteins and neuroapoptosis in the cerebral cortex in the APPswe/PSldE9 double transgenic mice with Alzheimer＇s disease （AD） induced by isoflurane. Methods Sixmonth-old AD mice and wild-type mice at the same age were randomly divided into wile-type control group, AD group, isoflurane group, electro-acupuncture group （N = 8）. The mice were given pretreatment with electro-acupuncture at Baihui（GV20） acupoint and Yougquan（KI1 ） acupoint once a day for 3 successive days, 15 rain each time. And then the mice in electro-acupuncture group and isoflurane group were exposed to a box full of 1.2% isoflurane for 4 hours. Morris water maze was used to test the learning and memmy abilities of the mice, Western blotting method was used to detect the expression of APP-C83, APP-C99 and Aβ, and terminal deoxynucleotidyl transferase （TdT） -mediated dUTP nick end labeling （TUNEL） was used to detect neuroapoptosis in the cerebral cortex. Results The escape latency of AD group was longer than that of wild-type mice group（P 〈 0.05） , and the latency of isoflurane group was longer than that of AD group （P 〈 0.05） , while the latency of electro-acupuncture group was shorter than that of isoflurane group （P 〈 0.05 ）. The percentage of retention time in the target quadrant and the times for crossing the target quadrant in isoflurane group were lower than those of AD group （P 〈 0.05）, but were higher in electro-acupuncture group than those in isoflurane group（P 〈 0.05）. APP- C83 expression level in isoflurane group was significantly lower than that in AD group （P 〈 0.05 ）, while APP-C83 expression level in electro-acupuncture group was higher than that in isoflurane group （P 〈 0.05）. APP-C99 expression level in isoflurane group was significantly higher than that in AD group （P 〈 0.05）, and APP-C99 expr

关 键 词：阿尔茨海默病/针灸疗法 细胞凋亡 异氟醚 Β淀粉样蛋白 Β淀粉样前体蛋白 疾病模型 动物 小鼠 

分 类 号：R245.97[医药卫生—针灸推拿学]