骨碎补总黄酮促进大鼠牵张成骨新骨形成的影像学研究  

Tomography Study on Total Flavonoids from Rhizoma Drynariae in Promoting New Bone Formation in Distraction Osteogenesis Rat Model

作　　者：姜自伟[1] 曾景奇 黄枫[1] 王凡 李悦[1] 虎群盛 曾志奎[1] 余翔[1] 朱海峰[1] JIANG Zi-Wei ZENG Jing-Qi HUANG Feng WANG Fan LI Yue HU Qun-Sheng ZENG Zhi-Kui YU Xiang ZHU Hai-Feng(The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou510405Guangdong, China Hunan Provincial Hospital of Traditional Chinese Medicine, Changsha 410005 Hunan, China)

机构地区：[1]广州中医药大学第一附属医院,广东广州510405 [2]湖南省中医院,湖南长沙410005

出　　处：《广州中医药大学学报》2017年第3期390-393,共4页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81403413)

摘　　要：【目的】建立SD大鼠牵张成骨模型,从影像学角度探索骨碎补总黄酮对大鼠牵张过程中骨形成质量的影响。【方法】24只大鼠造模成功后,随机分为实验组和对照组,分别给予骨碎补总黄酮(剂量为77.125 mg/kg)及生理盐水灌胃8周,术后7 d,以0.2 mm速度牵张20 d,术后8周拍片,处死取材并行标本Micro-CT扫描,观察2组X线Lane-Sandhu评分差别,并比较2组骨密度及骨矿含量。【结果】实验组X线Lane-Sandhu评分,Micro-CT扫描骨密度及骨矿含量均显著升高,与对照组比较,差异均有统计学意义(P<0.05)。【结论】骨碎补总黄酮可以提高大鼠牵张成骨过程中骨形成的质量。Objective To establish a distraction osteogenesis SD rat model for exploring the effect of total flavonoids from Rhizoma Drynariae on the quality of rat bone formation in the process of distraction osteogenesis from the perspective of tomography. Methods Twenty-four rats were randomly divided into experimental group and control group after successful modeling, and were respectively given intragastric administration with total flavonoids from Rhizoma Drynariae （in dose of 77.125 mg/kg） and saline for 8 weeks. Seven days after operation, the tibias of all the rats were given distraction at the speed of 0.2 mm for 20 days. Eight weeks after the operation, X-ray examination for all the rats was performed, and then the rats were killed for obtaining the tibia samples for Micro-CT scanning. The differences of X-ray Lane-Sandhu scores, the bone mineral density and bone mineral content were compared between the two groups. Results The X-ray Lane-Sandhu scores, the bone mineral density and bone mineral contents showed by Micro-CT scanning in the experimental group were higher than those in the control group, and the differences were statistically significant （P 〈 0.05）. Conclusion The total flavonoids from Rhizoma Drynariae can improve the quality of bone formation in the process of distraction osteogenesis in rats.

关 键 词：牵张成骨 骨碎补总黄酮 影像学 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]