姜黄素对三阴乳腺癌模型小鼠肿瘤的抑制作用及机理研究  

Anti-tumor Effect of Curcumin on Triple-negative Breast Cancer Mouse Model and its Mechanism

作　　者：林文新[1] 区俊文[2] 孙晓生[3] 彭磷基[2] 黄丽平[4,5] LIN Wen-Xin OU Jun-Wen SUN Xiao-Sheng PENG Lin-Ji HUANG Li-Ping(Guangzhou Institute of Dermatology, Guangzhou 510095 Guangdong, China Dept. of Oncology, Clifford Hospital, Guangzhou University of Chinese Medicine, Guangzhou 511495 Guangdong, China Basie Medieine Sehool, Guangzhou University of Chinese Medicine, Guangzhou 510006 Guangdong, China Hainan Medical University, Haikou 571199 Hainan, China Lingnan Normal Univeristy, Zhanjiang 524048 Guangdong, China)

机构地区：[1]广州市皮肤病防治所,广东广州510095 [2]广州中医药大学祈福医院肿瘤科,广东广州511495 [3]广州中医药大学基础医学院,广东广州510006 [4]海南医学院,海南海口571199 [5]岭南师范学院,广东湛江524048

出　　处：《广州中医药大学学报》2017年第3期404-408,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：海南省自然科学基金资助项目(编号:20168266);海南医学院科研培育基金资助项目(编号:H Y 2015-01);海南省科协青年科技人才学术创新计划项目(编号:H A ST201635)

摘　　要：【目的】研究姜黄素对三阴乳腺癌模型小鼠肿瘤的抑制作用及其机理。【方法】将人乳腺癌细胞MDA-MB-231接种到每只小鼠胸部皮下脂肪垫各0.1 m L(约1×106个细胞),移植瘤接种后第2天可见接种部位有结节,成瘤率100%。将40只造模成功的小鼠随机分为姜黄素低、中、高剂量组(剂量分别为10、20、40 mg/kg)和模型对照组,每组10只,连续给药30 d。给药结束后眼眶静脉丛采血并分离血清检测肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)的含量,分离出脾脏称重并计算脏器系数,记录瘤体质量和检测瘤体B淋巴细胞瘤-2(Bcl-2)基因和caspase-3的蛋白表达。【结果】与模型对照组比较,姜黄素各剂量组的caspase-3表达都显著增加(P<0.05或P<0.01),TNF-α含量、IL-6含量、瘤体质量、脾脏指数和Bcl-2表达显著减少(P<0.05或P<0.01)。【结论】姜黄素通过促进乳腺癌细胞凋亡对三阴乳腺癌模型小鼠肿瘤有一定的抑制作用。Objective To observe the anti-tumor effect and mechanism of curcumin on triple-negative breast cancer mouse model. Methods Human breast cancer ceils MDA-MB-231 were inoculated to the chest subcutaneous fat pad of mice（about 1 million cells in 0.1 mL cell suspension）. At day 2 after tumor inoculation, nodules were seen at inoculation site（tumor formation rate being 100%）. Forty successfully-modeled mice were randomly divided into low-, middle-, and high-dose curcumin groups （10, 20, 40 mg/kg） and model control group, 10 mice in each group. The medication lasted for 30 continuous days. After medication, the blood was taken out from the orbital venous plexus, and the serum was separated for the detection of tumor necrosis factor alpha （TNF- cx） and interteukin-6 （IL-6） contents. Spleen was separated for the calculation of organ coefficient, tumor mass was weighed, and the expression levels of Bcl-2 and caspase-3 were detected by Western blotting method. Results Compared with the model control group, caspase-3 expression level was significantly increased in the three cureumin groups（P 〈 0.05 or P 〈 0.01） , but the contents of TNF-α and IL-6, tumor weight, spleen index and Bcl-2 expression were significantly decreased（P 〈 0.05 or P 〈 0.01 ）. Conclusion Curcumin has inhibitory effect on triple-negative breast cancer mouse model through promoting the apoptosis of breast cancer cells.

关 键 词：姜黄素 乳腺癌/中药疗法 细胞凋亡 炎症因子 疾病模型 动物 小鼠 

分 类 号：R285.5[医药卫生—中药学]