中药脉平片质量控制标准的提高  

Improving quality standard of Maipingpian

作　　者：高进贤[1] 张秀娟[2] 呼延丽[1] 高栓生[1] 朱菊花[1] 葛斌[1] 

机构地区：[1]甘肃省人民医院药剂科,甘肃兰州730000 [2]甘肃省人民医院质控科,甘肃兰州730000

出　　处：《兰州大学学报（医学版）》2017年第2期37-42,共6页Journal of Lanzhou University（Medical Sciences）

摘　　要：目的通过进一步完善脉平片成分的测定,建立更全面的质量控制标准。方法采用薄层色谱法(TLC)对处方中银杏叶提取物进行定性鉴别,并对大黄素、大黄素甲醚的TLC法进行改进;采用高效液相色谱法(HPLC)对脉平片中总黄酮醇苷进行定量测定。结果银杏内酯A、银杏叶提取物、大黄素、大黄素甲醚的薄层色谱特征斑点分离清晰,阴性对照无干扰;槲皮素在0.029—0.588μg范围内所呈的线性关系良好,r=0.999 7;山柰素在0.062—0.411μg范围内所呈的线性关系良好,r=0.999 8;异鼠李素在0.057—0.566μg范围内所呈的线性关系良好,r=0.999 8。槲皮素平均加样回收率为97.22%,相对标准偏差(RSD)=1.28%;山柰素平均加样回收率为98.15%,RSD=1.40%;异鼠李素平均加样回收率为97.16%,RSD=1.47%。结论 TLC法和HPLC法操作简便、结果准确、重复性好,可用于控制脉平片的质量。Objective To establish a quality standard for Maipingpian, a Chinese medicine. Methods Thin- layer chromatography （TLC） was applied to the identification of Ginkgo biloba leaves （EGb）, and TLC of era- odin and physcion was improved. Total flavonol glycosides determined by high performance liquid chroma- tography （HPLC） was used with a photo-diode array detector. Results TLC of Ginkgolide A, EGb, emodin and physcion had distinct characteristic spots of separation and there was no interference in the negative com- parison. The linear range of quercetin was between 0.029-0.588 μg （r=0.999 7）; Kaempferol was between 0.062-0.411μg （r=0.999 8）; Isorhamnetin was between 0.057--0.566 μg （r=0.999 8）.The mean recovery of quercetin was 97.22%, RSD=1.28%; Kaempferol was 98.15%, RSD=1.40%; Isorhamnetin was 97.16% and RSD=1.47%. Conclusion TLC and HPLC are accurate, reliable and simple in operation, which can be used for the quality control of Maipingpian.

关 键 词：脉平片 银杏叶提取物 总黄酮醇苷 薄层色谱法 高效液相色谱法 

分 类 号：R453.9[医药卫生—治疗学]