和胃安神方对失眠模型大鼠OrexinA表达的影响  

Effect of Hewei Anshen Decoction(和胃安神方) on Orexin A Levels in Insomnic Rats

作　　者：张强 李绍旦[2] 刘毅[2] 曹科[2] 杨明会[2] 

机构地区：[1]海军北京莲宝路军职以上干部休养所,北京100036 [2]解放军总医院中医科,北京100853

出　　处：《中医药导报》2017年第8期21-24,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(81173429);首都医学发展基金(SF-2009-Ⅱ-16)

摘　　要：目的:探讨和胃安神方对失眠模型大鼠下丘脑食欲素A(OrexinA)的影响。方法:将100只雄性Wistar大鼠随机分为正常对照组、模型组及和胃安神方低、中、高剂量组。采用放射免疫分析法检测各组大鼠下丘脑组织OrexinA的含量,采用免疫组化法观察下丘脑OrexinA的表达。结果:模型组大鼠下丘脑OrexinA含量高于正常对照组,差异有统计学意义(P<0.01);和胃安神方低剂量组OrexinA含量低于模型组,但差异无统计学意义(P>0.05);和胃安神方中、高剂量组OrexinA含量均低于模型组(P<0.01);和胃安神方中、高剂量组OrexinA含量与正常对照组比较,差异无统计学意义(P>0.05)。和胃安神方中、高剂量组大鼠下丘脑OrexinA阳性细胞数目、积分光密度(IOD)均低于模型组,差异有统计学意义(P<0.01)。结论:和胃安神方可下调失眠模型大鼠下丘脑食欲素A(OrexinA)含量;和胃安神方治疗失眠症的机制可能与调节下丘脑OrexinA水平有关。Objective： To study the effect of Hewei Anshen Decoction （和胃安神方, HWASD） on orexinA levels in hypothalamus of insomnic rats. Methods： A hundred male Wistar rats were randomly divided into five groups： normal control group, insomnia model group, HWASD low dose group, HWASD middle dose group and HWASD high dose group. The content of orexinA in hypothalamus was measured by radioimmunoassav. The expression of orexinA positive neurons was detected by immunohistochemistry. Results： OrexinA levels in hypothalamus was significantly higher in insomnia model group than that in nomal group （P〈0.01）. After treatment, orexinA levels was lower in HWAS low dose group than that in insomnia model group, but the difference was not statistically significant （P〉0.05）. OrexinA levels were significantly lower in HWAS middle dose group and HWAS high dose group than that in insomnia model group （P〈0.01）, and was not statistically different with normal control group （P〉O,05）. Compared with insomnia model group, IOD of orexinA positive neurons was significantly lower in HWAS middle dose group and high dose group （P〈0.01）. Conclusion： The therapeutic effect of HWASD on insomnia is partially attributed to decreased content and expression of orexinA in hypothalamus.

关 键 词：失眠 和胃安神方 下丘脑 食欲素A 大鼠 

分 类 号：R285.5[医药卫生—中药学]