双参通冠方对心肌梗死大鼠HIF-1α和VEGF表达的影响  

Effects of Shuangshen Tongguan Decoction(双参通冠方) on HIF-1α and VEGF Expression in Rat Model with Myocardial Infarction

作　　者：王永刚[1,2] 李彩红[1,2] 于远望[1] 钟伟[1,2] 齐婧[1,2] 尚俊平[1,2] 

机构地区：[1]陕西中医药大学心脑血管病研究所,陕西咸阳712000 [2]陕西中医药大学第二附属医院,陕西咸阳712000

出　　处：《中医药导报》2017年第8期25-27,34,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：陕西省科技厅自然科学研究计划项目(2015JM8384);陕西省中医药管理局中医药科研项目(15-SCJH017);陕西省第五批重点专科心脑血管病科建设项目;国家中医药管理局重点专科心血管病科建设项目

摘　　要：目的:研究双参通冠方对急性心肌梗死大鼠缺血心肌内皮细胞VEGF及上游调控因子HIF-1α表达的影响,探讨双参通冠方防治心肌梗死的作用机制。方法:SD大鼠分为模型组、空白组、双参通冠方低剂量组(2.5 g/kg)、中剂量组(5 g/kg)、高剂量组(7.5 g/kg),采用结扎左冠状动脉前降支建立大鼠心肌梗死模型,灌胃给药2周后,取大鼠的心肌组织,制备心肌组织匀浆,Elisa法检测VEGF含量,Western blot法检测VEGF及上游调控因子HIF-1α的表达。结果:模型组大鼠心肌组织VEGF含量和VEGF、HIF-1α表达高于空白组,差异有统计学意义(P<0.05);双参通冠方低剂量组、中剂量组和高剂量组VEGF含量和VEGF、HIF-1α表达均高于模型组,差异均有统计学意义(P<0.05);双参通冠方低剂量组VEGF含量和VEGF、HIF-1α表达低于双参通冠方中剂量组,双参通冠方高剂量组VEGF含量和VEGF、HIF-1α表达高于双参通冠方中剂量组,差异均有统计学意义(P<0.05)。结论:双参通冠方可增加急性心肌梗死模型大鼠缺血区心肌组织中VEGF含量和上游调控因子HIF-1α表达,促进血管内皮细胞增殖,诱导缺血部位血管新生,对心肌具有保护作用。Objective： To study the effects of Shuangshen Tongguan Decoction （双参通冠方, SSTGD） on VEGF in ischemic myocardial endothelial cells and the upstream regulatory factors HIF- expression, then to explore the mechanism of SSTGD prevention and treatment of myocardial infarction. Methods： The SD rats were divided into model group, the control group, the SSTGD low dose group （2.5 g/kg）, middle dose group （5 g/kg）, and high dose group （7.5 g/kg）. Use the ligation of left anterior descending coronary artery to establish myocar- dial infarction model in rats, to give medicine for 2 weeks. Fetch the myocardial tissue in rat, preparation of myocardial tissue homogenate, use Elisa method to detect the contents of VEGF, western blot method to detect the expression of VEGF and the upstream regulatory factors HIF-1α. Results： Compared with the control group, model group rats myocardial tissue VEGF levels and the VEGF, HIF-lα expression increased （P〈0.05）; Com- pared with model group, the SSTGD low dose group, middle dose group and high dose group VEGF content and the VEGF, HIF-1α expression increased, the difference was statistically significant （P〈0.05）; Compared with SSTGD middle dose group, low dose group of VEGF content and the VEGF, HIF-1α expression decreased, high dose group of VEGF content and the VEGF, HIF-1α expression increased, difference was statistically significant （P〈O.05）. Conclusion： The SSTGD could increase content of VEGF in ischemic myocardial tissue and upstream regulatory factors HIF-lα expression, and promote vascular endothelial cell proliferation, inducing ischemia parts angiogenesis for myocardial protection.

关 键 词：双参通冠方 心肌梗死 VEGF HIF-1Α 大鼠 

分 类 号：R285.5[医药卫生—中药学]