HPLC法测定山药饮片及麸炒山药中尿囊素的含量  

Determination of Allantion in Dioscorea Opposita and Rhizoma Dioscoreae by HPLC

作　　者：焦锐锐 王成永[1] 

机构地区：[1]安徽中医药大学药学院,安徽合肥230001

出　　处：《安徽卫生职业技术学院学报》2017年第2期105-106,109,共3页Journal of Anhui Health Vocational & Technical College

基　　金：国家科技基础性工作专项课题(编号:2014FY111100-10)

摘　　要：目的:建立测定山药饮片及麸炒山药中尿囊素的方法,测定山药饮片及麸炒山药中尿囊素的含量。方法:采用phenomenex Gemini 5u C18 110A(250×4.6mm)为色谱柱,流动相为甲醇-水(1∶99),流速为1.0mL/min,柱温为30℃,检测波长为212nm。结果:该方法在2~50ug/mL范围内与峰面积呈良好的线性关系,r值为0.9996;加样回收率为96.33%,RSD为1.32%(n=6)。结论:该方法条件稳定,操作简单,精密度与回收率也较好,结果准确,可靠,能为山药饮片的质量评价提供依据。Objective：To establish a method for Determination of Allantion in Dioscorea Opposita and Rhizoma Dioscoreae and determination content of allantion.Method：separation and determination of allantion were accessed by using phenomenex Gemini 5u C 18 110A（250 × 4.6ram ） column and a mobile phase of methanol-water at a ratio of 1 ： 99（v/v）.The flow rate was 1.0mL/min,temperature at 30 ℃ and the detection wavelength at 212nm.Result：A good linearity of allantion in the rang of 2-50ug,r=0.9996;the mean value of recovery is 96.33%,RSD is 1.32%（n=6）. Conclusion：The established method is stable,simple ,accurate with good recovery and can provide an evidence for quality control of Dioscorea Opposita.

关 键 词：山药 麸炒山药 尿囊素 高效液相 含量测定 

分 类 号：R446.5[医药卫生—诊断学]