新型鼻腔填塞材料聚乙二醇双丙烯酸酯复合物水凝胶的细胞相容性  

Cytocompatibility of a novel nasal packing material made of polyethylene glycol diacrylate composite hydrogel

作　　者：杨国慧[1] 韩德民[1] 臧洪瑞[1] 李立峰[1] 

机构地区：[1]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科耳鼻咽喉头颈科学教育部重点实验室(首都医科大学),北京市100730

出　　处：《中国组织工程研究》2017年第10期1546-1551,共6页Chinese Journal of Tissue Engineering Research

基　　金：北京市医院管理局"使命"人才计划项目(SML20150201)~~

摘　　要：背景:前期研究发现聚乙二醇双丙烯酸酯和壳聚糖形成的复合物可生物降解吸收,具有抗炎消肿能促进创面愈合作用,且生物相容性好,然而该复合物质地较脆,鉴于鼻腔解剖结构,需要一种具有力学支撑性能的材料来克服这种缺陷。目的:观察新型可降解吸收鼻腔填塞材料聚乙二醇双丙烯酸酯复合物水凝胶的细胞相容性。方法:通过光交联将聚乙二醇双丙烯酸酯、壳聚糖、聚乙烯醇材料复合,形成聚乙二醇双丙烯酸酯复合物水凝胶,根据国际通行医疗器械生物学评价标准采用直接解触法及间接浸提法评价其细胞相容性。(1)直接解触法:将聚乙二醇双丙烯酸酯复合物水凝胶与小鼠成纤维细胞L929共培养24 h,观察形态变化;(2)间接浸提法:分别以100,50μL聚乙二醇双丙烯酸酯复合物水凝胶浸提液及100μL RPMI1640培养液培养小鼠成纤维细胞L929,CCK-8法检测培养1-3 d的增殖及细胞毒性。结果与结论:(1)直接解触法:材料周围细胞形态呈伴突起的梭形或星形的扁平状结构,贴壁性生长,细胞饱满且折光性强,整体呈生长趋势,可见该材料生物相容性良好;(2)间接浸提法:随着时间的增加,含复合物水凝胶浸提液组细胞数量增加,细胞相对增殖率逐渐增加,细胞毒性为0至1级;(3)结果表明:聚乙二醇双丙烯酸酯复合物水凝胶材料具有良好的细胞相容性。BACKGROUND： Previous studies have shown that the complex of polyethylene glycol diacrylate and chitosan is biodegradable, has the anti-inflammation effect and can promote wound healing, with good biocompatibility.However, the composite material is brittle, and thus, it is necessary to develop a mechanical support material due to the anatomy of the nasal cavity.OBJECTIVE： To study the cytocompatibility of a novel degradable absorbable nasal packing material made of polyethylene glycol diacrylate composite hydrogel.METHODS： In our experiment, the composite hydrogel was formed by the combination of different ratios of polyethyleneglycol diacrylate, chitosan and polyvinyl alcohol using photo-crosslinking method. According to the international standard of medical devices, we used direct and indirect international standard experimental methods to evaluate the cytotoxicity of the materials. （1） Direct contact method： the polyethylene glycol acrylate composite hydrogel and mouse fibroblasts L929 were co-cultured for 24 hours to observe the morphological changes of cells. （2） Indirect extraction： mouse fibroblasts L929 were cultured in polyethylene glycol diacrylate composite hydrogel extracts （100, 50 μL） and RPMI1640 medium （100 μL）, respectively, for 3 days to observe the proliferation and cytotoxicity by cell counting kit-8 assay.RESULTS AND CONCLUSION： （1） Direct contact method： the cells around the material formed a flat structure with fusiform or stellated protrudes. The material was confirmed to have good biocompatibility depending upon the presence of adherent cell growth, full and strong refraction, and the overall growth trend. （2） Indirect extraction method： with the increase of time, the number of cells in the hydrogel extract group increased, the relative proliferation rate of cells increased gradually, and the cytotoxicity was 0 to 1. To conclude, these experimental findings indicate that the polyethylene glycol acrylate composite hydrogel material has good cytocomp

关 键 词：生物材料 材料相容性 聚乙二醇双丙烯酸酯水凝胶 鼻填塞材料 细胞毒性 小鼠成纤维细胞 

分 类 号：R318[医药卫生—生物医学工程]